Stuying phylogenetic groups of Escherichia isolated from poultry feces based on genes chu, yio, tspe isolated from Kerman province

Document Type : Full Research Paper

Authors

1 M., Department of Microbiology, Sirjan Branch, Islamic Azad University, Sirjan, Iran.

2 Assistant Professor, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran.

3 Assistant Professor, Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.

4 Assistant Professor, Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran.

Abstract

Escherichia coli is a gram negative enteric bacteria and the causative agent of important diseases in poultry and humans and has zoonotic importance. This bacteria is divided into four phylogenetic groups including A1, B1, B2 and D and more subgroups which has epidemiologic importance. The aim of study is to evaluate the prevalence of phylogenetic groups of E. coli strains isolated from poultry feces. In this study 60 samples of E. coli isolated from poultry were collected and confirmed by culture and biochemical tests. Triplex-PCR was done on the isolates and by chuA, yjaA genes and TspE4.C2 to determine the phylogenetic groups. The results of the study showed the phylogroup B2 was the most group identified and the subgroups A0, A1, B1, B22, B23, D1 and D2 were observed in 18.3%, 8.3%, 1.6%, 5%, 41.6%, 3.3% and 16.6% of the isolates. This study showed the most strains of E. coli isolated from feces of poultry belonged to B2 phylogenetic group which is an exteraintestinal pathogen group in humans and shows the zoonotic importance of these isolates.

Keywords

Main Subjects


1. Ghanbarpour, R., Daneshdoost, S. (2012). Identification of Shiga toxin and intimin coding genes in Escherichia coli isolates from pigeons (Columba livia) in relation to phylotypes and antibiotic resistance patterns. Trop Anim Health Prod‎;‎ 44(2), 307-312.
2. Carvalho, A. C., A. V. Barbosa, L. R. Arais, P. F. Ribeirob, V. C. Carneiro, A. M. F. Cerqueir. (2016). Resistance patterns, ESBL genes, and genetic relatedness of Escherichia coli from dogs and owners; Braz J Microbiol; 47: 150–158.
3. Clermont, O., S. Bonacorsi, and E. Bingen. (2000). Rapid and simple determination of the Escherichia coli phylogenetic group; Appl Environ Microbiol; 66: 4555-4558.
4. Clermont, O., J. K. Christenson, E. Denamur and D. M.Gordon. (2013). The Clermont Escherichia coli phylotyping method revisited: improvement of specificity and detection of new phylo-groups; Env Microbiol Reports; 5: 58-65.
5. Jeong, Y. W., Kim, T. E., Kim, J. H., & Kwon, H. J. (2012). Pathotyping avian pathogenic Escherichia coli strains in Korea. J vet sci‎;‎ 13(2), 145-152.
6. Derakhshandeh, A., R. Firouzi and Z Naziri. (2014). Phylogenetic group determination of faecal Escherichia coli and comparative analysis among different host; IJVR; 15( 1): 13-17.
7. Duriez, P., O. Clermont, S. Bonacorsi, E. Bingen, A. Chaventré, J Elion, B. Picard and E. Denamur. (2001). Commensal Escherichia coli isolates are phylogenetically distributed among geographically distinct human populations; Microbiology; 147: 1671-1676.
8. Desjardins, P., B. Picard, B. Kaltenbock, J. Elion and E. Denamur. (1995). Sex in Escherichia coli does not disrupt the clonal structure of the population: evidence from random amplified polymorphic DNA and restrictionfragment-length polymorphism; J MolEvol; 40: 440-448.
9. Derakhshandeh, A., Firouzi, R., Moatamedifar, M., Motamedi, A., Bahadori, M., Naziri, Z. (2013). Phylogenetic analysis of Escherichia coli strains isolated from human samples. Mol Biol Res Communi‎;‎ 2(4), 143-149.
10. Ewers, C., E. M. Antao, I. Diehl, H. C. Philipp and L. H. Wieler. (2009). Intestine and environment of the chicken as reservoirs for extraintestinal pathogenic Escherichia coli strains with zoonotic potential; Appl Env Microbiol; 75(1): 184–192.
11. Ghanbarpour, R. and M. Salehi. (2010). Virulence genotyping of Escherichia coli isolates from avian cellulitis in relation to phylogeny; Comp Clin Pathol; 19(2): 147–153.
12. Gordon, D. M. (2004). The influence of ecological factors on the distribution and genetic structure of Escherichia coli. In Escherichia coli and Salmonella typhimurium. American Society for Microbiology.
13. Herzer, P. J., S. Inouye, M. Inouye and T. S. Whittam. (1990). Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli; J Bacteriol; 172: 6175-6181.
14. Hussein, A. H. M., I. A. I. Ghanem, A. A. M. Eid, M. A. Ali, J. S. Sherwood, G. Li, L. K. Nolan and C. M. Logue. (2013). Molecular and phenotypic characterization of Escherichia coli isolated from broiler chicken flocks in Egypt; Avian Dis; 57(3): 602-611.
15. Liu, Y., Liu, G., Liu, W., Liu, Y., Ali, T., Chen, W., Han, B. (2014). Phylogenetic group, virulence factors and antimicrobial resistance of Escherichia coli associated with bovine mastitis. Res Microbiol‎;‎ 165(4), 273-277.
16. Johnson, J. R., P. Delavari, M. Kuskowski and A. L. Stell. (2001). Phylogenetic distribution of extraintestinal virulence-associated traits in Escherichia coli; J Infect Dis; 183: 78-88.
17. Lecointre, G., L., Rachdi, P. Darlu and E. Denamur. (1998). Escherichia coli molecular phylogeny using the incongruence length difference test; Mol Biol Evol; 15: 1685-1695.
18. Martins, R. P., da Silva, M. C., Dutra, V., Nakazato, L., da Silva Leite, D. (2013). Preliminary virulence genotyping and phylogeny of Escherichia coli from the gut of pigs at slaughtering stage in Brazil. Meat sci‎;‎ 93(3),437-440.
19. KarimiDarehabi, H., M. H. Naseri, S. Menbari, J. Mobaleghi and E. Kalantar. (2013). Antibiotic resistance patterns of Escherichia coli groups A, B1, B2 and D isolated from frozen foods and children with diarrhea in Sanandaj, Iran; Int J Enter Pathog; 1(1): 1-4.
20. Kazemnia, A., M. Ahmadi and M. Dilmaghani. (2014). Antibiotic resistance pattern of different Escherichia coli phylogenetic groups isolated from human urinary tract infection and avian colibacillosis; Iran Biomed J; 18 (4): 219-224.
21. Nakhaee, P., S. M. Peighambari and J. Razmyar. (2015). Phylogenetic group determination of Escherichia coli isolated from broilers and layers with colibacillosis; Iran J Vet Sci Tech; 7( 1): 12-21.
22. Sabarinath, A., K. P. Tiwari, C. Deallie, G. Belot, G. Vanpee, V. Matthew, R. Sharma and H. Hariharan. (2011). Antimicrobial resistance and phylogenetic groups of commensal Escherichia coli isolates from healthy pigs in Grenada. Webmed Central Veterinary Medicine 2(5): 1942.
23. Hungerford, T. G. (1961). Respiratory diseases of poultry. Aust Vet J‎;‎ 37(4), 93-96.
24. Walk, S. T., E. W. Alm, EW, L. M. Calhoun, J. M. Mladonicky and T. S. Whittam. (2007). Genetic diversity and population structure of Escherichia coli isolated from freshwater beaches; Environ Microbiol; 9: 2274-2288.