Stuying phylogenetic groups of Escherichia isolated from poultry feces based on genes chu, yio, tspe isolated from Kerman province

Document Type : Full Research Paper

Authors

1 M., Department of Microbiology, Sirjan Branch, Islamic Azad University, Sirjan, Iran.

2 Assistant Professor, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran.

3 Assistant Professor, Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.

4 Assistant Professor, Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran.

Abstract

Escherichia coli is a gram negative enteric bacteria and the causative agent of important diseases in poultry and humans and has zoonotic importance. This bacteria is divided into four phylogenetic groups including A1, B1, B2 and D and more subgroups which has epidemiologic importance. The aim of study is to evaluate the prevalence of phylogenetic groups of E. coli strains isolated from poultry feces. In this study 60 samples of E. coli isolated from poultry were collected and confirmed by culture and biochemical tests. Triplex-PCR was done on the isolates and by chuA, yjaA genes and TspE4.C2 to determine the phylogenetic groups. The results of the study showed the phylogroup B2 was the most group identified and the subgroups A0, A1, B1, B22, B23, D1 and D2 were observed in 18.3%, 8.3%, 1.6%, 5%, 41.6%, 3.3% and 16.6% of the isolates. This study showed the most strains of E. coli isolated from feces of poultry belonged to B2 phylogenetic group which is an exteraintestinal pathogen group in humans and shows the zoonotic importance of these isolates.

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References

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History

  • Receive Date: 17 December 2016
  • Revise Date: 23 July 2017
  • Accept Date: 25 July 2017

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