Molecular detection of avian infectious bronchitis serotypes in broiler chicken flocks with respiratory clinical signs in Guilan province

Document Type : Full Research Paper

Authors

1 Veterinary Research Department, Gilan Agricultural and Natural Resources Research and Education Center, AREEO, Rasht, Iran.

2 Iran Veterinary Organization, Guilan Provincial Veterinary Service, Rasht, Iran

3 Iran Veterinary Organization, Guilan Provincial Veterinary Service, Rasht, Ira

4 Rahimabadi, E., Gilan Agricultural and Natural Resources Research and Education Center, AREEO, Rasht, Iran.

5 Department of Research and Diagnosis of Poultry Disease Razi Vaccine and Serum Research Institute , AREEO, karaj, Iran.

Abstract

Avian infectious bronchitis (IB) is caused by corona viruses. Infectious bronchitis virus (IBV) is a major cause of economic losses in poultry and can be involved in respiratory disease, nephritis, and both poor egg production and quality by reproductive tract infection. IBV has many serotypes that do not confer cross protection against each other. The aim of this study was conducted to detect IBV serotypes in broiler chicken farms of Guilan province were involveled with clinical signs respiratory disease. In the present study, tracheal and lung tissue samples were collected randomly from 28 broiler chicken farms with respiratory signs. Reverse Transcription Polymerase Chain Reaction (RT-PCR) method was done after extraction of RNA virus from samples for diagnosis of IBV. 20 flocks (70%) out of 28 flocks were positive. Nested- PCR method was performed for detection of 3 serotypes of IBV (Mass, 793/B and D274) by using of specific primers. The results showed that 13samples (65%) belonged to Mass serotype, 7 samples(35%) to 793/B and both of Mass, 793/B serotypes were detected from 2 samples (10%), but in all of samples D274 serotype was not detected. In conclusion, for the best immunity against IB in broiler flocks of Guilan province, using of 2 serotypes (Mass, 793/B) in vaccination programs with biosecurity in farms are recommended.

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References

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Veterinary Research & Biological Products
Volume 30, Issue 4
December 2017
Pages 260-267

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History

  • Receive Date: 24 June 2017
  • Revise Date: 12 July 2017
  • Accept Date: 15 July 2017

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