Survey of effects of different levels of dietary macroalgae (Gracilaria pygmaea) on some hematological parameters Asian Sea bass (Lates calcarifer)

Document Type : Full Research Paper

Authors

1 Masters Graduated of Fisheries, University of Persian Gulf, Bushehr, Iran.

2 Assistant Professor of Persian Gulf Research Center, University of Persian Gulf, Bushehr, Iran.

3 Associate Professor of Faculty of Agriculture and Natural Resources, University of Persian Gulf, Bushehr, Iran.

4 Research Expert Institute of the Persian Gulf, Persian Gulf University, Bushehr, Iran.

5 PhD student, the Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran.

Abstract

Almost 300 species of marine algae has been identified in south of Iran that they were classified in groups of red algae (Rhodophytes), brown algae (Phaeophytes) and green algae (Chlorophytes). Among seaweed, Gracilaria made due to high production and extraction of economic value such as agar, one of the most important algae. Graclaria algae are Gracilariaceae family and red algae (Rhodophyta) category. The aim of this study was to investigate the effect of algae powder as additives in the diet Grasilaria Asian Sea bass (Lates calcarifer) on hematological parameters were. For this purpose, 120 Asian sea bass fish with an average weight of 28 ± 8/1g in 4 treatments and 3 replications in twelve 300-liter fiberglass tanks (10 fish per tank) was divided. The treatments based on each of the four diets containing 0, 1.5, 2 and 2.5 percent algae powder Gracilaria per kg food was prepared. At the end of the experiment the fish tail vein stem collected blood samples and blood parameters were measured. The results of this study indicated that supplementation of the macroalgae didn’t influence the blood parameters including hemoglobin, hematocrit, white blood cells count and differential WBC counts in Asian sea bass.

Keywords

References

1. Ahmadifar, E., Jalali, M.A., sudagar, M., Azari Takami, Gh., Mahmmadi Zarj Abadi, A. 2009. Effects of Aqua Vac Ergosan on the growth performance, survival and haematological factors in Beluga (Huso Huso) juvenile. Journal of Agricultural Sciences and Natural Resources Vol. 16 (Special issue1-a) (In Persian).
2. Amar, E.C., Kiron, V., Satoh, S., Watanabe, T. 2004. Enhancement of innate immunity in rainbow trout (Oncorhynchus mykiss) associated with dietary intake of carotenoids from natural products. Fish and Shellfish Immunology 16(4) P: 57–37.
3. Andrews, S.R., Sahu, N.P., Pal, A.K., Mukherjee, S.C. Kumar, S. 2011. Yeast extract, brewer's yeast and spirulina in diets for (Labeo rohita) fingerlings affect haemato-immunological responses and survival following (Aeromonas hydrophila) challenge. Research in Veterinary Science 91 P: 103-109.
4. AOAC, 2005. Official Method of Analysis 17th (end), Washington. DC: Association of Official Analytical Chemists.
5. Araújo, M., Rema, P., Sousa-Pinto, I., Cunha, L.M., Peixoto, M. J., Pires, M.A., Seixas, F., Brotas, V., Beltrán, C., Valente, L.M.P. 2016. Dietary inclusion of IMTA-cultivated Gracilaria vermiculophylla in rainbow trout (Oncorhynchus mykiss) diets: effects on growth, intestinal morphology, tissue pigmentation, and immunological response. Journal of Applied Phycology 28(1) P: 679–689.
6. Barros, M.M., Lim, C., Klesius, P.H. 2002. Effect of iron supplementation to cottonseed meal diets on growth performance of channel catfish, (Ictalurus punctatus). Aquaculture 10 P: 65-86.
7. Blaxhall, P.C., Daisley, K.W. 1983. Routine hematological methods for use fish with blood. Journal of Fish Biology 5 P: 771-781.
8. Chelemal Dezfol Nezhad, M.,Jahangirizadeh, M., Mesbah, M., Javaheri Baboli, M. 2011. The effect of feeding with Spirulina (Spirulina platensis) on the blood and immune system in fish Pangasius (Pangasius hypophtalamus). New Journal of Veterinary Research 2 (7) P: 1-9 (In Persian).
9. Feist, G., Van Enennaam, J.P., Doroshov, S.I., Schreck, C.B., Scheider, R.P. 2004. Early indentification of sex in cultured white sturgeon (Acipenser transmontanus) using plasma steroid levels. Aquaculture 232: 581-590.
10. Ghasemi Pirbalooti, A., Pirali, A., Pishkar, Gh., Jalali, S.M.A., Raeisi, M., Jaafarian, D., Hamedi, B. 2011. The effect of some pharmaceutical plant essence on Rainbow trout (Oncorhynchus mykiss) immunological system. Journal of Herbal Drugs 2(2): 149-155 (In Persian).
11. Gudding, R., Lillehaug, A., Evensen, Q. 1999. Recent development in fish vaccinology. Vetrinary Imonology and Immunopathology 72 P: 203-212.
12. Heidarieh, M., Mirvaghefi, A.R., Akbari,M., Farahmand, H., Sheikhzadeh, N., Shahbazfar, A.A., Behgar, M. 2012. Effect of dietary Ergosan on growth performance, digestive enzymes, intestinal histology, hematological parameters and body composition of rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem 38 P:1169–1174.
13. Hedayati, S.A.A., Bagheri, T., Yavari, V., Bahmani, V., Alizadeh, M. 2008. Check some blood biochemical parameters Beluga in brackish water (Huso Huso). Journal of Marine Biology 21 (4) P: 658-666 (In Persian).
14. Jalali, M.A., Ahmadifar, E., Sudagar, M., AzariTakami, Gh. 2009. Growth efficiency, body composition, survival and hematological changes in great sturgeon (Huso Huso Linnaeus, 1758) juveniles fed diets supplemented with different levels of Ergosan. Aquaculture Research 40 P: 804-809.
15. Khadjeh, G.H., Peyghan, R. 2007. Evaluation of some blood serrum biochemical parameters of rainbow trout (Onchorhynchus mykiss) cultured in earthen ponds. Journal of Veterinary Research 62(3) P: 197-203 (In Persian).
16. Mahmoudi, N., Abdi, H., Falahatcar, B. 2010. The effect of dietary nucleotides on hematological and biochemical indices of common carp (Cyprinus carpio). Journal of Marine Science and Technology 9(3) P: 4-12 (In Persian).
17. Morshedi, V., AQ, N., Morammezi, J., Nori, F., Mohammadian, T. 2015. Zayloolygosakarid prebiotic effects of diet and nutrition on growth performance, blood and answers (Sparidentex hasta) fry non-specific Sobaity. Journal of Marine Biology. Islamic Free University of Ahvaz 7 (26) P: 69-82 (In Persian).
18. Morshedi, V., AQ, N., Morammezi, J., Nori, F., Mohammadian, T. 2015. Lactoferin effects of diet and nutrition on growth performance, blood and answers (Sparidentex hasta) fry non-specific Sobaity. Journal of Animal Environment 8 (2) P: 181-190 (In Persian).
19. Otatake, M., Kiryu, I., Nakanishi, T. 2002. Development of vaccine delivery method for fish: Parcutaneous administration by immersion with application of multiple puncture instruments. Journal of Vaccine 1 P: 3764-3769.
20. Peixoto, M.J., Salas-Leitón, E., Pereira, L.F., Queiroz, A., Magalhães, F., Pereira, R., Abreu, H., Reis, P.A., Gonçalves, J.F.M., de Almeida Ozório, R.O. 2016. Role of dietary seaweed supplementation on growth performance, digestive capacity and immune and stress responsiveness in European seabass (Dicentrarchus labrax). Aquaculture Reports 3 P: 189–197.
21. Pourgholam, Y., Khara, H., Mohseni, M.2014. The effects of different levels of vitamin C on blood safety and Siberian sturgeon (Acipenser baerii). Journal of Physiology and Development of Animal 35 P: 79-85 (In Persian).
22. Rabei, R., Asadi, M., Nejadsattari, T., Majd, A., Sohrabipour, J. 2007. The study of species diversity in association of (Gracilaria salicornia) in northeast of Qeshm Island. Pajouhesh and Sazandegi 66 P: 85-92 (In Persian).
23. Rahimi Bashar, M., Tehrani Fard, A., Ghaseminejad, A., Alipur., Fallahchai, D. 2008. Determine some blood parameters Whitefish Caspian Sea (Rutilus frissii kutum) at different stages of gonadal development. Journal of Biology Science P: 45-56 (In Persian).
24. Razeghi Mansur, M., Akrami, R., Ghobadi, Sh., Amani Dengi, K., Shoaii, R. 2012. Effect of dietary mannan oligosaccharide on some parameters Hematological and serum biochemical beluga Young farmed (Huso Huso Linnaeus, 1754). Iran Veterinary Journal 2 P: 12-21 (In Persian).
25. Rehulka, J. 2000. Influence of astaxanthin on growth rate. Condition and some blood indices of rainbow- trout. Aquaculture 190 P: 27-47. https://doi.org/10.1016/S0044-8486(00)00383-5
26. Rios, F.S., Kalinin, A.L., Rantin, F.T. 2002. The effects of long term food deprivation on respiration and haemato long of the neotropical fish Hoplias malabaricus. Journal of fish Biology 61 P: 85-95.
27. Salighe Zadeh, R., Yavari, V., Mousavi, S.M., Zakeri, M. 2014. Effects of dietary supplement (Spirulina platensis) on blood, immunological and serum biochemical parameters benny fish (Mesopotamichthys sharpeyi) (Günther, 1874) Iranian Veterinary Journal 2(10) P: 41-47 (In Persian).
28. Shahidi yasaghi, S.A., Mazandarani, M., ghorbani Hasan Saraei, A., ghorbani, R., Soleimani, N. 2008 Determination of normal values of some blood serum factors (Electrolyte and non electrolyte) of Acipenser persicus. Fisheries Magazine P: 32-25 (In Persian).
29. Shapawi, R., Zamry, A.A. 2016. Response of Asian sea bass, (Lates calcarifer) juvenile fed with different seaweed-based diets. Journal of Applied Animal Research 44 P: 121-125.
30. Sakai, M. 1999. Current research status of fish immunostimulants. Aquaculture 172 P: 63–92.
31. Trono, J.R., Gavino, C. 1997. Field guide and Atlas of the Seaweed Resources of The Philippines P. 306.
32. Welker, T.L., Lim, C., Yildirim-Aksoy, M., Shelby, R., Klesius, P.H. 2007. Immune response and resistance to stress and (Edwardsiella ictaluri), fed diets containing commercial whole-cell yeast or yeast subcomponents. Journal of World. Aquaculture Society 38(1) P: 24-35.
33. Zaman Nezhad, N., Emadi, H., Hossain Zadeh Sahafi, O. 2015. The effect of nutritional algae (Sargassum illicifolium) on the change of immunoglobulin levels (IgM), and lysozyme in rainbow trout (Onchorhynchus mykiss). Journal of Marine Science and Technology10 (4) (In Persian).
Veterinary Research & Biological Products
Volume 30, Issue 4
December 2017
Pages 223-230

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History

  • Receive Date: 21 December 2016
  • Revise Date: 01 January 2017
  • Accept Date: 01 January 2017

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