Comparison of virulence and histopathological changes of NC-1 and NC-SweB1 isolates of Neospora caninum in in chicken embryonated eggs

Document Type : Full Research Paper

Authors

1 Veterinary Faculty, Islamic Azad University, Karaj, Iran.

2 Department of Pathobiology, Veterinary Faculty, Islamic Azad University, Karaj, Iran

3 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.

Abstract

Inspite of limited data on the role of birds in the field of infection with Neospora caninum, there is no report about the role of chicken in the field of infection with this parasite. In this study, two isolates of NC-1 and NC-SewB1 in Ross embryonated eggs were used. For each of the isolates, six different dilutions (10, 102 ,103,104,105,106) were prepared for infection. Group 7 was considered as control group. Insemination was conducted in chorioallantoic liquid of embryonated eggs in 9 days. Rates of death, macroscopic changes, and tissue histopathology of dead chicken and chickens that were kept one month after hatching were evaluated. The results showed that the mortality rate in both NC-1 and NC-SweB1 was dependent on amount of inseminated tachyzoite. Thus, in greater quantities of tachyzoite, rates of death and histopathological lesions were higher. The comparison of death of embryonated eggs in isolated NC-1 and NC-SweB1 both using Fisher test showed that the total mortality rate in both groups was statistically significant. According to the average lethal dose (LD50) that was 101.5 and 101.1 for NC-1 and NC-SweB1, respectively, we can conclude that virulence of the NC-1 is higher than NC-SweB1. The results of this study showed that the study of embryonated eggs can be a valuable approach to test live and attenuated vaccines in the in vivo environments.

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 4
December 2017
Pages 92-99

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History

  • Receive Date: 31 October 2016
  • Revise Date: 24 December 2016
  • Accept Date: 08 January 2017

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