Health monitoring of Razi Institute laboratory mice (NIH strain) to Clostridium piliforme in 1395

Document Type : Full Research Paper

Authors

1 Member of Scientific Board, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

2 MSc,Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

The cause of tyzzer's disease is Clostridium piliforme bacteria. Since the international standards on determination of contamination in laboratory animals in Iran It's not done.This study intends review the incidence of Clostridium piliforme infection in laboratory NIH mice colonies in a laboratory animals breeding center. 82 mice from a colony of breeding laboratory animals were randomly selected and were examined by indirect ELISA method for specific antibodies against Clostridium piliforme in serum. Also after necropsy the gastrointestinal were studied examined for symptoms of the disease. Of these, two positive cases were identified in ELISA and any symptoms of disease was not observed in necropsy. According to the FELASA instructions, it can be concluded with 99.9% confidence, the contamination with Clostridium piliforme in breeding facilities, much less than 10% (2.43%) in the NIH mice colony is obtained and is not need to remove the colony. However, should be considered carefully and be more sensitive to this bacteria in periodic testing.

Keywords

References


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Veterinary Research & Biological Products
Volume 30, Issue 4
December 2017
Pages 78-84

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History

  • Receive Date: 24 January 2017
  • Revise Date: 25 February 2017
  • Accept Date: 03 April 2017

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