Study on Experimental Infection of Newcastle Disease Virus in Local Breeder Chicks Infected with Infectious Bursal Disease Virus

Document Type : Full Research Paper

Authors

1 Avian Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz-Iran.

2 Avian Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz-Iran

Abstract

Clinical signs, macroscopic lesions, virus tissue distribution, shedding and humoral immune responses to virulent Newcastle disease virus in native breeder chicks were studied .One hundred and twenty day-old native breeder chicks were divided randomly into four equal groups. Groups one and two were infected with 103 CID50 infectious bursal disease virus (IBDV) by intra-bursal route on day-old. Groups one and three chicks were infected with 105EID50 virulent ND virus by nasal route on 30th days. Fourth group 4 chicks were kept as uninfected control group. Clinical signs were observed daily after each inoculation. Tracheal and cloacal swabs from four live birds of each group collected on days of two, 5, 10, 15 and 18 post NDV infection and on the same days except days eighteen, four chicks were randomly euthanized and organ tissue samples from brain, trachea, lung, kidney and liver were collected to detect ND virus by RT-PCR method. Clinical signs was more severe in IBDV and ND virus infected group and ND virus was detected from trachea and cloaca swabs for a longer period and mean HI titer to ND virus in these birds was lower. Two-way analysis of variance showed IBD infection significantly affected immune humoral response (P<0.001). The study showed that infection of native breeder chicks with IBDV increased bird sensitivity, ND pathogenicity and virus shedding.

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 4
December 2017
Pages 11-23

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History

  • Receive Date: 04 November 2016
  • Revise Date: 15 December 2016
  • Accept Date: 28 December 2016

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