Evaluation of Balb/c mice estrous cycle stages

Document Type : Short Communication

Authors

Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran​

Abstract

Reproductive cycle in rodents is known as estrous cycle. In rodents, the duration of the estrous cycle is shorter than other mammals. The shortened estrous cycle made rodents as a suitable animal model to evaluate the impact of drug treatment, nutritional effect, and stress on reproductive cycle. In bread mouse like BALB/c showed significant decrease in case of reproductive efficiency. The aim of the present study was to find simple and reliable method for detection of different stages of estrous cycle in rodents, especially in mice. One of the proposed methods in estrous cycle monitoring would be the conventional vaginal sampling. In the present study, this method has been modified. To conduct the study we use 10 mice. Mice had ad libitum accessed to water and food. The light cycle was 12:12 (L/D). All the samples were collected between 9-11 A.M. Sampling was performed using 100 µL sampler and physiological slain solution. Results of the present study revealed that a combination of three different cell types were detectable during Pro-estrous (nuclear epithelial cells, cornified epithelial cells), Estrous (cornified epithelial cells), Met-estrous (cornified epithelial cells, and leucocytes), and Di-estrous (leucocytes).

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 3 - Serial Number 116
September 2017
Pages 274-279

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History

  • Receive Date: 20 June 2016
  • Revise Date: 12 July 2016
  • Accept Date: 13 July 2016

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