Histological and histochemical study of urinary system of native partridges (Alectoris chukar)

Document Type : Full Research Paper

Author

Department of Anatomical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.

Abstract

In order to determine the histological and histochemical structure of the urinary system, this study was carried out in 10 female and 10 male twenty-week-old healthy native partridges. The birds were anesthetized by chloroform inhalation and euthanized. After dissection, the position of kidneys and ureters with related structures studied. Tissue samples from cranial, middle and caudal portions of each kidney and ureter fixed in 15% neutral buffered formalin. Sections stained with H&E for histological observation and special stainings: Masson’s trichrome, Verhoeff’s, PAS, Alcian blue, and Gomori’s for histochemical investigation. Two nephron types present in kidney of birds. Renal capsule was made up of all the connective tissue fibers. Alcian blue and PAS-positive reaction found in the proximal convoluted tubules, collecting tubules and collecting ducts. The primary branches of ureter composed of tunica mucosa, submucosa, muscularis and serosa, whereas the secondary branches was only consisted of epithelium and connective tissue fibers. In all different regions of ureter, lymphatic tissues were not observed in the lamina propria. Histology of kidney and ureter showed no significant sex-based differences. Also, the histological structures of the kidney showed no significant differences among different divisions of the left and right kidneys. The microvilli and mucosal folds were more identified in the proximal region than those of the other regions of the organ. The thickness of tunica muscularis increased from the proximal to the distal region of the ureter. Unusual findings of the ureter in Alectoris chukar were the presence of isometric longitudinal folds, and muscularis mucosa.

Keywords

References

1- Abdul-Gahaffor, R., A. Al-Ajeely and S. M. Fadhil. 2012. Morpho-histological study on the development of kidney and ureter in hatching and adulthood racing pigeon (Columba livia domestica). International Journal of Science and Nature 3: 665-677.
2- Al-Azawy, N. H. 2005. Comparative anatomical and histological study of kidney in domestic fowls and geese. MSc Thesis, Baghdad University. Baghdad, Iraq.
3- Aughey, E. and F. L. Frye. 2001. Comparative Veterinary Histology with Clinical Correlates. 1st ed. pp. 143-148. Manson Publishing, London.
4- Bacha, W. J. and L. M. Bacha. 2012. Color Atlas of Veterinary Histology. 3rd ed. pp. 184-185. Wiley Blackwell, Philadelphia.
5- Casotti, G. and E. J. Braun. 2000. Renal anatomy in sparrows from different environments. Journal of Morphology 243: 283-291.
6- Casotti, G., K. K. Lindberg and E. J. Braun. 2000. Functional morphology of the avian medullary cone. American Journal of Physiology; Regulatory, integrative and comparative physiology 279: 1722-1730.
7- Casotti, G. 2001. Effects of season on kidney morphology in house sparrows. Journal of Experimental Biology 204: 1201-1206.
8- Dayani, A. 1988. The Birds of Middle East and near east. 1st ed. pp. 90-100. University of Tehran, Tehran.
9- Dhyaa, A. A., F. R. Ali, S. K. Azhar and A. A. Myson. 2014. Comparative anatomical and histological features of the kidney in harrier (Circus aueroginosus), chicken (Gallus domesticus) and mallard duck (Anas platyrhynchos). Iraqi Journal of Veterinary Medicine 38: 107-113.
10- Echols, M. S. 2006. Evaluating and treating the kidneys. pp. 451-491, In: G. J. Harrison and T. L. Lightfoot (ed.), Clinical Avian Medicine. Vol. 2, Spix Publishing, Palm Beach (FL).
11- Goldstein, D. L. and E. Skadhauge. 2000. Renal and extrarenal regulation of body fluid composition. pp. 265-297, In: G. C. Whittow (ed.), Sturkie’s Avian Physiology. Academic Press, San Diego.
12- Islam, K. N., M. Z. Khan, M. S. Siddiqui, M. R. Islam, N. S. Lucky, M. K. Hossain and G. N. Adhikary. 2004. The anatomical studies of the kidneys of Rhode Island Red (RIR) and White Leghorn chicken (WLH) during their postnatal stages of growth and development. International Journal of Poultry Science 3: 369-372.
13- Kiernan, J. A. 2015. Histological and histochemical methods: theory and practice. 5th ed. pp. 502. Scion, Bloxham.
14- Lafi Batah, A. 2012. Morphological and histological study for the kidneys of coot bird (Fulica atra). Basra Journal of Veterinary Research 11: 128-136.
15- Lierz, M. 2003. Avian renal disease: pathogenesis, diagnosis and therapy. Veterinary Clinics Exotic Animal Practice 6: 29–55.
16- McNabb, A., R. A. McNabb and H. R. Steeves. 1973. Renal mucoid materials in pigeons fed high and low protein diets. Ornithological Advances 90: 14-18.
17- Mehrabani Yeganeh, H. 1994. The History and evolution of the domestic fowl. Kosar economic organization.
18- Mirabella, N., V. Esposito, M. Corona and G. V. Pelagalli. 2007. The morphology of the ureter in the duck (Anas platyrhynchos). Anatomia Histologia Embryologia 27: 237-243.
19- Mobini, B. 2016. Histological and anatomical study of trachea of native partridges (Chukar chukar). Pajouhesh & Sazandegi 112: 2-9.
20- Mobini, B., and M. H. Abdollahi. 2016. Effect of sex on histological and histochemical structures of different parts of the kidney in Japanese quail. Poultry Science 95: 2145-2150.
21- Nabipour, A., E. Alishahi and M. Asadian. 2009. Some histological and physiological features of avian kidney. Journal of Applied Animal Research 36: 195-198.
22- Rezaian, M., A. Sodagar Amiri, M. Ebrahim Akbari and F. Ebrahimpoor. 2007. Modification of fixation in preparing avian tissues. Pajouhesh & Sazandegi 74: 2-8.
23- Zohari, M. A. 2003. Poultry meat production. 11th ed. pp. 225. University of Tehran, Tehran.
Veterinary Research & Biological Products
Volume 30, Issue 3 - Serial Number 116
September 2017
Pages 111-121

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History

  • Receive Date: 14 October 2016
  • Revise Date: 03 November 2016
  • Accept Date: 05 November 2016

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