Association between blood metabolites, tumor necrosis factor alpha concentrations and metabolic disorders in Holstein cows during transition period

Document Type : Full Research Paper

Authors

Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

Abstract

The objectives of present study was to evaluate the association among blood metabolites, tumor necrosis factor (TNF)-alpha and metabolic diseases in Holstein cows during transition period. Pregnant multiparous Holstein cows (N = 50), with body condition scores (BCS) 3.5±0.75 and third parity were selected. Blood samples were collected weekly from 2 week prior to calving to 2 weeks after calving to determine plasma concentrations of non-esterifies fatty acids (NEFA), beta-Hydroxyl butyrate acid (BHBA) and activity of aspartate aminotransferase (AST) and alanine aminotrans-ferase (ALT) enzymes. According to above parameters cows divided into healthy (N =10) and diseases cow (n= 10) and plasma concentrations of glucose, insulin and TNF-α compared weekly from 2 week prior to calving to 2 weeks after calving.Results showed cows with metabolic disorders had higher plasma concentrations of NEFA, BHBA, AST and TNF-alpha compared to healthy cows (P < 0.05) but conversely plasma concentrations of glucose and insulin were lower in cows with metabolic disorders compared to healthy cows (P < 0.05). Plasma glucose concentration affected by time and increased from -14 to calving in both treatments and after that decreased until d +14. Glucose concentrations at d +7 and +14 were greater in healthy cows compared to cows with metabolic disorders. Plasma TNF-α concentration were greater in cows with metabolic disorders compared to healthy cows during whole esxperiment. In conclusion due to greater TNF-α concentration in cows with metabolic disorders before calving, using this item could be useful to predict cows suspicious to metabolic disorders after calving therefore we can use suitable strategies to prevent or decreased incidence of metabolic disorders.

Keywords

References

1. Ametaj BN, Bradford BJ, Bobe G, Nafikov RA, Lu Y, Young JW and Beitz DC (2005) Strong relationships between mediators of the acute phase response and fatty liver in dairy cows. Canadian Journal of Animal Science 85: 165-175.
2. As AN, Nazifi S, Ghasrodashti AR and Olyaee A (2011) Prevalence of subclinical ketosis in dairy cattle in the Southwestern Iran and detection of cutoff point for NEFA and glucose concentrations for diagnosis of subclinical ketosis. Preventive Veterinary Medicine 100: 38-43.
3. Bernabucci U, Ronchi B, Lacetera N and Nardone A (2005) Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science 88: 2017-2026.
4. Bertoni G, Trevisi E, Han X and Bionaz M (2008) Effects of inflammatory conditions on liver activity in puerperium period and consequences for performance in dairy cows. Journal of Dairy Science 91: 3300-3310.
5. Bradford BJ, Mamedova LK, Minton JE, Drouillard JS and Johnson BJ (2009) Daily injection of tumor necrosis factor-α increases hepatic triglycerides and alters transcript abundance of metabolic genes in lactating dairy cattle. Journal of Nutrition 139: 1451-1456.
6. Brickner AE, Pires JA, Gressley TF and Grummer RR (2009) Effects of abomasal lipid infusion on liver triglyceride accumulation and adipose lipolysis during fatty liver induction in dairy cows. Journal of Dairy Science 92: 4954-4961.
7. Drackley JK (1999) Biology of dairy cows during the transition period: the final frontier? Journal of Dairy Science 82: 2259-2273.
8. Galvão KN, Santos NR, Galvão JS and Gilbert RO (2011) Association between endometritis and endometrial cytokine expression in postpartum Holstein cows. Theriogenology 76: 290–299.
9. García-Ruiz I, Rodríguez-Juan C, Díaz-Sanjuan T, del Hoyo P, Colina F, Muñoz-Yagüe T and Solís-Herruzo JA (2006) Uric acid and anti-TNF antibody improve mitochondrial dysfunction in ob/ob mice. Hepatology 44: 581-591.
10. Hammon DS, Evjen IM, Dhiman TR, Goff JP and Walters JL (2006) Neutrophil function and energy status in Holstein cows with uterine health disorders. Veterinary Immunology Immunopatholohy 113: 21–29.
11. Ingvartsen KL (2006). Feeding-and management-related diseases in the transition cow: Physiological adaptations around calving and strategies to reduce feeding-related diseases. Animal Feed Science and Technology 126: 175-213.
12. Jiang L, Sorensen P, Rontved C, Vels L and Ingvartsen K (2008) Gene expression profiling of liver from dairy cows treated intra-mammary with lipopolysaccharide. BMC Genomics 9: 443.
13. Joksimovic Todorovic M and Davidovic V (2013) Immunosuppression postpartum diseases of dairy cow. Biotechnology Animal Husbandry 29: 211-222.
14. Kasimanickam RK, Kasimanickam VR, Olsen JR, Jeffress EJ, Moore DA and Kastelic JP (2013) Associations among serum pro- and anti-inflammatory cytokines, metabolic mediators, body condition, and uterine disease in postpartum dairy cows. Reproductive Biology and Endocrinology 11: 103-107.
15. Kushibiki S, Hodate K, Shingu H, Obara Y, Touno E, Shinoda M and Yokomizo Y (2003) Metabolic and lactational responses during recombinant bovine tumor necrosis factor-α treatment in lactating cows. Journal of Dairy Science 86: 819-827.
16. Lacetera N, Scalia D, Bernabucci U, Ronchi B, Pirazzi D and Nardone A (2005) Lymphocyte functions in over conditioned cows around parturition. Journal of Dairy Science 88: 2010-2016.
17. Melendez P, Donovan GA, Risco CA and Goff JP (2004) Plasma mineral and energy metabolite concentrations in dairy cows fed an anionic prepartum diet that did or did not have retained fetal membranes after parturition. American Journal of Veterinary Research 65: 1071-7076
18. Rajala-Schultz PJ, Grohn YT and McCulloch CE (1999) Effects of milk fever, ketosis and lameness on milk yield in dairy cows. Journal of Dairy Science 82: 288–294.
19. Sharma N, Singh NK, Singh OP, Pandey V and Verma PK (2011) Oxidative stress and antioxidant status during transition period in dairy cows. Journal of Animal Science 24: 479-484.
20. Spears JW and Weiss WP (2008) Role of antioxidants and trace elements in health and immunity of transition dairy cows. The Veterinary Journal 176: 70-76.
21. Stojević Z, Piršljin J, Milinković-Tur S, Zdelar-Tuk M and Ljubić BB (2005) Activities of AST, ALT and GGT in clinically healthy dairy cows during lactation and in the dry period. Veterinarski Archive 75: 67-73.
22. Trevisi E, Amadori M, Bakudila AM and Bertoni G (2009) Metabolic changes in dairy cows induced by oral, low-dose interferon-alpha treatment. Journal of Animal Science 87: 3020-3029.
23. Turk R, Juretic D, Geres D, Svetina A, Turk N, et al. (2008) Influence of oxidative stress and metabolic adaptation on PON1 activity and MDA level in transition dairy cows. Animal Reproduction Science 108: 98-106.
Veterinary Research & Biological Products
Volume 30, Issue 3 - Serial Number 116
September 2017
Pages 147-153

Files

History

  • Receive Date: 22 October 2016
  • Revise Date: 01 November 2016
  • Accept Date: 01 November 2016

Share

How to cite

Statistics