Study of Pediococcus pentosaceus effect on histomorphometrical structure of intestine in juvenile rainbow trout (Oncorhynchus mykiss Walbaum , 1792)

Document Type : Full Research Paper

Authors

1 Department of basic sciences, Faculty of veterinary medicine, University of Tehran, Tehran, Iran.

2 Department of Fisheries, Talesh Branch, Islamic Azad University, Talesh, Iran.

3 Department of Aquatic Animal Health, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.

4 Faculty of veterinary medicine, University of Tehran, Tehran, Iran.

Abstract

In recent decades, many studies have been conducted evaluating the effects of probiotics in fish. Its positive effects on growth performance and the health of fish have been reported. Rainbow trout is one of the aquaculture species which is important in economic aspect. Juvenile rainbow trout were used in one control group (without bacteria in food) and three treatment groups with108 CFU/g Pediococcus pentosaceus in different doses of 200,300,400 g/ 1000 kg food were used in this study. After eight weeks, tissue samples from different parts of the intestine have been taken and then fixed in 10 % formalin. Then, tissue passages were stained with hematoxylin-eosin, and Masson’s trichrome. The results showed that histometrical values like height and width of the villi in anterior and middle intestine of treatment groups especially in 400 g bacteria / 1000 kg food was significantly (p<0.05) increased in comparison with that of control group. It seems that Pediococcus pentosaceus caused positive effects on proliferation and growth of the intestine tissue which lead to increase of absorption surface area and growth of the juvenile rainbow trout.

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 3 - Serial Number 116
September 2017
Pages 232-245

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History

  • Receive Date: 25 August 2016
  • Revise Date: 17 October 2016
  • Accept Date: 18 October 2016

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