Effects of Escherichia coli Lipopolysaccharide on Chicken Humoral Immune Response

Document Type : Full Research Paper

Authors

1 Department of Pathobiology, Veterinary Faculty of Shahid Chamran University. Ahvaz, Iran.

2 Department Clinical Science, Veterinary Faculty of Shahid Chamran University. Ahvaz, Iran.

3 Department of Pathobiology, Veterinary Faculty of Shahid Chamran University. Ahvaz, Iran

4 Student of Veterinary faculty of ShahidChamran University of Ahvaz, Iran.

Abstract

In this research, extraction, purification and inactivation of Escherichia coli (E. coli) lipopolysaccharide (LPS) were performed with the aim of investigating the effects of inactivated and intact form of LPS on chicken humoral immune response stimulation. Also, the possibility of application of this outer part of cell wall for specific induction of humoral immune response to its reference bacteria was evaluated. The LPS was extracted using Ethylene diamine tetraacetic acid (EDTA). Four doses (10, 25, 50 and 100 µg) of inactivated and intact form of LPS and 100 µL of %20 sheep red blood cell (SRBC) were injected to eight groups of broiler chickens (N=4). The humoral immune responses were evaluated five and 10 days after treatment by haemagglutination assay. The effects of LPS on induction of humoral immune response to the reference bacteria were measured using in-house ELISA. Injection of 10 and 25 µg of LPS significantly (P<0.05) induced the humoral immune response. The inactivated LPS only induced immune responses 10 days after the injection of 100 µg. No significant difference existed between the various groups in antibody titer against E. coli (P<0.05). It is possible to take advantage from Escherichia coli lipopolysaccharide in poultry immunization. Also, evaluation of the side effects and changes of inactivation methods may show the capability of LPS application in animal immunization. In addition, using LPS as a vaccine for E. coli is not suggested.

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References


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Veterinary Research & Biological Products
Volume 30, Issue 3 - Serial Number 116
September 2017
Pages 71-77

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History

  • Receive Date: 20 August 2016
  • Revise Date: 11 October 2016
  • Accept Date: 15 October 2016

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