The Study of Suppressive Effects of Pasteurella multocida B:2 on Calf Lymphocytes

Document Type : Full Research Paper

Authors

1 Asisstant Prefossor, Department of Poultry Bacterial Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organisation (AREEO), Tehran, Iran

2 Asisstant Prefossor, Department of Poultry Bacterial Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organisation (AREEO), Tehran Iran

3 Student BSc, Scientific Biology Faculty, Kharzmi University, Karaj, Iran.

Abstract

Haemorrhagic Septicaemia (HS) is an acute disease of cattle and buffaloes in tropical countries, caused by Pasteurella multocida (P. multocida) serotype B:2 , a Gram-negative coccobacillus. Some evidences of immunosuppression have been observed after a challenge with a virulent strain on vaccinated calves. To further investigate the observed suppressive effect, the present study was set up using a cell-free extract and calf PBMCs, for in vitro experiments. A lymphocyte stimulation assay was used to assess the effects of a cell-free extract (CFE) of P. multocida on peripheral blood mononuclear cells (PBMCs), isolated from calves. To assess the proliferative response of PBMCs obtained from calf, ConA was added to the PBMCs culture, and the proliferation was quantified by assessment of radioactivity of absorbed 3H-Thymidine. In order to characterize the agent(s), the CFE extract suppressive activity was assessed after heating and dialyzing and also by using an outer-membrane protein (OMP) preparation in experiments. Results indicated that addition of CFE at 50 μg/ml caused a five fold decrease in the proliferative response to ConA. Heating at 80˚C for five min completely destroyed the suppressive properties. An outer membrane protein (OMP) preparation of P. multocida B:2 markedly suppressed the proliferative response of PBMCs to ConA. The study was indicative of a suppressive component(s) of P. multocida B:2 on proliferative response of calf PBMCs to ConA. The active part of the suppressive agent(s) was likely to be at least in some part of protein, and is of OMPs.

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References

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Veterinary Research & Biological Products
Volume 30, Issue 3 - Serial Number 116
September 2017
Pages 53-62

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History

  • Receive Date: 01 June 2016
  • Revise Date: 03 September 2016
  • Accept Date: 06 September 2016

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