Histological and histochemical study of kidney in Guinea fowl (Numida meleagris)

Document Type : Full Research Paper

Authors

1 Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Professor of Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

The kidneys are important organs that regulate blood pressure and the volume and composition of body fluids. The most important part of the kidney is nephron that removes waste materials from the body. Because, there was little information on histology and histochemistry of kidney in Guinea fowl, this study was performed on five specimens. After preparation of the specimens were anesthetized and their kidneys were removed along with their synsacrum bone and were placed in 10% buffered formalin solution for 24 hours. Then, the kidneys were separated from the bone and tissue sections were prepared and stained with Hematoxylin – eosin and PAS-Alcian Blue stains. The results showed that epithelium of proximal, distal and cortical collecting tubules was simple cuboidal. The most part of kidney cortex was surrounded with proximal tubules. The epithelium of thick and thin limbs of Henle’s loop was composed of cuboidal so that it was short cuboidal in thin limb and high cuboidal in thick limb. The epithelium of medullary collecting tubules was high simple cuboidal to columnar. In histochemistry results, the apical border of distal and collecting tubules cells was stained with Alcian Blue, and the apical border of proximal tubule cells was stained with PAS and they were differentiated from each other.

Keywords

References

1- ضمیری، م.ج. (1386). فیزیولوژی دام. چاپ چهارم، انتشارات حق شناس، صفحات: 265- 288.
2- Ayeni, J.S.O. and Ayanda, J.O. (1982).Studies of the husbandry practices and social acceptance of guinea fowl in Nigeria. Bulletin of Animal Health and Production in Africa, 30 (2):139-148.
3 -Canny, C. (1978). Gross anatomy and imaging of the avian and reptilian urinary system. Seminars in Avian and Exotic Pet Medicine, Vol 7, No 2: pp 72-80.
4 -Casotti, G. (2001). Effects of season on kidney morphology in house sparrows. The Journal of Experimental Biology. 204(6): 1201-6.
5 -Casotti, G. and Braun, E.J.(2000). Renal anatomy in sparrows from different environments. Journal of Morphology. 243(3):283-91.
6 -Casotti, G. and Richardson, K.C. (1993). A qualitative analysis of the kidney structure of Meliphagid honeyeaters from wet and arid environments. Journal of Anatomy.182(2): 239-47.
7- Hewitson, T.D. Darby (eds.), I.A. (2010). Histology Protocols, Methods in Molecular Biology 611.
8- Lafi, A. B. (2012).morphological and histological study for the kidney of Coot Bird (Fulica atra). Bas. J.Vet.Res.11.(1):128 -136.
9- McNabb, F.M.A. McNabb, RA. and Ward, JJM. (1972). The effects of dietary protein content on water requirements and ammonia excretion in pigeons, Columbia livia. Comparative Biochemistry and Physiology Part A Physiology 43:181–185.
10- Michaleh, K. Szczerbinska, D. Grabowska, M. Majewska, D. Laszczynaska, M. (2016). Anatomical and morphological study of the kidneys of the breeding emu (Dromaius novaehollandiae). 40: 314-319.
11- Nabipour, A. Alishahi, E. and Asadianm, M. (2009). Some Histological and Physiological Features of Avian Kidney. J. Appl. Anim. Res. 36: 195-198.
12- Nishimura, H. Koseki, C. Imai, M. and Braun, E.J. (1989). Sodium chloride and water transport in the thin descending limb of Henle of the quail. American Journal of Physiology. 257: 994-1002
13-Sabat, P., Maldonado, K., Canals, M. and Martınez, D. (2006). Osmoregulation and adaptive radiation in the ovenbird genus Cinclodes (Passeriformes: Furnariidae). Funictional Ecology. 20:799–805.
14- Singer, M.A. (2003). Do mammals, birds, reptiles and fish havesimilar nitrogen conserving systems? Comparative Biochemistry and Physiology Part B. 134:543–558.
15-Sivakumar, S.A., Ushakumary, S. and Hayath Basha, S. (2012). Microanatomical studies on the renal cortex of guinea fowl. Tamilnadu Journal of Veterinary & Animal Sciences 8 (1) 29-35.
16- Skadhauge, E. (1981). Osmoregulation in birds. Berlin, Heidelberg, New york: Springer – Verlag.
17- Ward, JM. McNabb, RA. McNabb, FMA. (1975a). Effects of changes in dietary protein and water availability on urinary nitrogen compounds in rooster,Gallus domesticus-I. Urine flow and excretion of uric-acid and ammonia. Comparative Biochemistry and Physiology. 51:165–169.
18- Ward, J.M., McNabb, R.A., McNabb, F.M.A. (1975b). The effects of changes in dietary protein and water availability on urinary nitrogen compounds in the rooster, Gallus domesticus-II. Diurnal patterns in urine flowrates, and urinary uric acid and ammonia concentrations. Comparative Biochemistry and Physiology. 51:171–174.
19- Wideman, R.F. (1988). Avian kidney anatomy and physiology. CRC Critical Rev. Poultry Biology. 1:133-176.
Veterinary Research & Biological Products
Volume 30, Issue 2
May 2017
Pages 167-173

Files

History

  • Receive Date: 29 October 2015
  • Revise Date: 17 June 2016
  • Accept Date: 17 June 2016

Share

How to cite

Statistics