Evaluation of attenuated variety of Neospora caninum for diagnosis of infection in cattle by agglutination test

Document Type : Full Research Paper

Authors

1 Student, Azad University of Jahrom Branch

2 Member of Board Scientific Razi Vaccine and Serum Research Institute, Shiraz Branch, Agricultural Research, Education and Extension Organisation (AREEO), Tehran, Iran.

Abstract

The protozoan parasite Neospora caninum is a major cause of abortion in cattle worldwide. It has been found in various animal species such as dogs, cattle, horses, sheep and goats. This is conclusive proof that long-term passage of tachyzoites in tissue culture can attenuate virulence of Neospora caninum in vivo. But whether the attenuation procedure could reduces the diagnostic value of attenuated variety is the issue of the impact that this study tries to evaluate it. In other word the aim of this study was to evaluate the attenuated variety of Neospora caninum for diagnosis of neosporosis in cattle by agglutination method. The study was conducted with 184 sera collected from cattle with a history of abortion during the past year from the farms of Fars province. The sera were tested for the detection of antibodies against Neospora caninum with commercial ELISA kit and agglutination method. The results of this study showed that the positivity rate for the sera was 54 (29.34%) for agglutination method compared to 59(32.07 %) obtained by ELISA kit. The difference of agglutination method with ELISA kits according to the McNemar test was not significant and this result was also very good agreement with the Kappa test. This study proved that the attenuated variety of Neospora caninum still preserves its own diagnostic value.

Keywords

References

1. Bartley, P.M., Wright, S., Sales, J., Chianini ,F., Buxton ,D ., and Innes, E.A.(2006). Long Term passage of tachyzoites in tissue culture can attenuate virulence of Neospora caninum Invivo. Parasitology, 133:421-432.
2. Bjo¨rkman, C., Alenius, S., Emanuelsson, U., Uggla, A., 2000. Neospora caninum and bovine virus diarrhea virus infections in Swedish dairy cows in relation to abortion. Veterinary Journal, 159:201–206.
3. Chamberland, S., Current, L.W., 1990. Use of mouse macrophage cell lines for in vitro propagation of Toxoplasma gondii RH tachyzoites. Proceedings of the Society Experimental Biology Medicine, 150–157.
4. Değirmenci A1, Döşkaya M, Caner A, Ciçek C, Korkmaz M, Gürüz Y, Uner A. Toxoplasma gondii RH Ankara: production of evolving tachyzoites using a novel cell culture method. Exp Parasitol. 2011 May;128(1):1-8.
5. Döskaya M1, Degirmenci A, Ciçek C, Ak M, Korkmaz M, Gürüz Y, Uner A. Behaviour of Toxoplasma gondii RH Ankara strain tachyzoites during continuous production in various cell lines. Parasitology. 2006 Mar;132(Pt 3):315-9. Epub 2005 Dec 1.
6. Dubey, J.P. Lindsay, D.S., Anderson, M.L., Davis, S.W., Shen, S.K. (1992). Induced transplacental transmission of Neospora caninum in cattle. J Am Vet Med Assoc, 201(5): 709-713.
7. Dubey, J.P., Buxton, D., and Wouda, W. (2006). Pathogenesis of bovine Neosporosis. Journal of Comparative Pathology, 134: 267-289.
8. Dubey, J.P., Zarnke, R., Thomas, N.J., Wong, S. K., Van Bonn, W., Briggs, M., Davis, J.W., Ewing, R., Mensea, M., Kwok, O.C.H., Romand, S., Thulliez, P., 2003. Toxoplasma gondii, Neospora caninum, Sarcocystis neurona, and Sarcocystis canis-like infections in marine mammals. Veterinary Parasitology, 116:275–296.
9. Hall, R., Ilhan, T., Kivar, E., Wilkie, G., Preston, P. M., Darghouth, M., Somerville, R. and Adamson, R. (1999). Mechanism(s) of attenuation of Theileria annulata vaccine cell lines. Tropical Medicine and International Health 4, A78–A84.
10. Hemphill, A., Vonlaufen, N., Naguleswaran, A., Keller, N., Riesen, M., Guetg, N., Srinivasan, S. and Alaeddine, F., 2009. Tissue culture and explant approaches to studying and visualizing Neospora caninum and its interactions with the host cell. Microscopy and Microanalysis, 10: 602–620.
11. Khordadmehr , M., Namavari ,M , Khodakaram-Tafti ,A., Mansourian, M., Rahimian, A., and Daneshbod, Y.(2013). Comparison of use of Vero cell line and suspension culture of murine macrophageto attenuation of virulence of Neospora caninum. Research in Veterinary Science, vol, 95 , pp:515–521.
12. Levine, N.D., 1988a. Progress in Taxonomy of the Apicomplexan Protozoa. Journal of Eukaryotic Microbiology 35, 518–520
13. Montoya, J.G., Liesenfeld, O., 2004. Toxoplasmosis. Lancet 363, 1965–1976
14. Namavari M, Hosseini MH, Mansourian M, Shams Z, Amrabadi O, Tahamtan Y, Moazeni-Jula F., Screening for causative infectious agents of abortion in Iran, Online JVet Res., 16 (3):147-153, 2012.
15. Namavari, M., Mansourian, M., Khodakaram-Tafti, A., Hosseini, M.H., Rahimiyan, A., Khordadmehr, M., Lotfi, M., 2011. Application of chicken embryonated eggs as a new model for evaluating the virulence of Neospora caninum tachyzoites. Comparative Clinical Pathology, 1346–1349.
16. Packham, A.E., Sverlow, K.W., Conrad, P.A. (1998). A Modifed Agglutination test for Neospora caninum: development, optimization, and comparison to the indirect fluorescent-antibody test and enzyme-linked immunosorbent assay. Clinical and Diagnostic Laboratory Immunology, 5: 467-473.
17. Ramamoorthy, S., Duncan, R., Lindsay, D.S. and Sriranganathan, N. (2007). Optimization of the use of C57BL/6 mice as a laboratory animal model for Neospora caninum vaccine studies. Veterinary Parasitology 145: 253-259
18. Razmi, G.R., Maleki, M., Farzaneh, N., Talebkhan Garoussi M. and Fallah, A.H.(2007). First report of Neospora caninum-associated bovine abortion in Mashhad area, Iran. Parasitology Research 100(4): 755-757
19. Reichel, M.P., Ellis, J.T., Dubey, J. P., 2007. Neosporosis and hammondiosis in dogs. Journal of Small Animal Practice, 48: 308–312.
20. Snow, R.W., Guerra, C.A., Noor, A.M., Myint, H.Y., Hay, S.I., 2005. The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature. Mar 10;434:214-7.
21. Suresh K1, Mak JW, Yong HS. 1991 Long term maintenance of Toxoplasma gondii (Rh strain) in Vero cell line and use of harvested antigens for immunodiagnosis. Southeast Asian J Trop Med Public Health. Dec;22 Suppl:124-8.
22. Wallach, M., 2010. Role of antibody in immunity and control of chicken coccidiosis. Trends in Parasitology 26, 382–387
23. Wilkins, M.F.,O’Connell,E., and TePunga,W.A. (1988).Toxoplasmosis in sheep III. Further evaluation of the ability of a live Toxoplasma gondii vaccine to prevent lamb losses and reduce congenital infection flowing an experimental oral challenge. New Zealand Veterinary Journal,36:86-89.
Veterinary Research & Biological Products
Volume 30, Issue 2
May 2017
Pages 153-157

Files

History

  • Receive Date: 03 July 2016
  • Revise Date: 26 July 2016
  • Accept Date: 26 July 2016

Share

How to cite

Statistics