The evaluation of antifungal effects of Althaea officinalis and Syzygium aromaticum aqueous extracts against Penicillium spp and Aspergillus spp isolates

Document Type : Full Research Paper

Authors

1 Department Pharmacology, Faculty of Veterinary Medicine, Shahrekord University, Iran. Sadeghi,

2 Department of Phararmacology, Faculty of Veterinary Medicine, Shahrekord University, Iran.

3 Department Pharmacology, Faculty of Veterinary Medicine, Shahrekord University, Iran.

4 Department Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Iran.

Abstract

Due to identification of antifungal drugs side effects and development of antifungal drugs-resistant fungi, there are growing interest to replace antifungal drugs with the herbal remedies that have antifungal properties. The present study was conducted to evaluate the antifungal effects of Althaea officinalis and Syzygium aromaticum aqueous extracts on the two species of saprophytic fungi including Penicillium spp and Aspergillus spp. Antifungal effects were assessed at four different concentrations including 6.25, 12.5, 25 and 50 mg/ml. The results of the present study revealed that Althaea officinalis and Syzygium aromaticum aqueous extracts have antifungal effects against Penicillium spp in all applied concentrations. Althaea officinalis aqueous extract had antifungal effects against Aspergillus spp at the concentration of 25 and 50 mg/ml, however, Syzygium aromaticum aqueous extract had an inhibitory effect on Aspergillus spp at all concentrations. The results demonstrate ameliorative effects of these herbal remedies on Penicillium spp and Aspergillus spp Furthermore, these results indicate that the antifungal effect of Syzygium aromaticum is greater than Althaea officinalis.

Keywords

References


1. Ali Shah, S. M., N. Akhtar, M. Akram, P. Akhtar Shah, T. Saeed, K. Ahmed, and H. M. Asif. 2011. Pharmacological activity of Althaea officinalis L. Journal of Medicinal Plants Research, 5(24):662-666.
2. Al-Snafi, A. E. 2013. The pharmaceutical importance of Althaea officinalis and Althaea rosea: a review. International Journal of PharmTech Research, 5(3): 1378-1385.
3. Ayatolahi Mousavi, S. A., H. Abdollahi and N. Kazemipour. 1996. Investigation of antifungal activity of 10 methanol extracts of medicinal herbs. Journal of Kerman University of Medical Sciences, 3(3): 115-122. (In Farsi).
4. Bennett, J. W. and M. Klich, 2003. Mycotoxins. Clinical Microbiolog Review, 16(3): 497-516.
5. Bluma, R. V. and M. G. Etcheverry. 2008. Application of essential oils in maize grain: impact on Aspergillus section Flavi growth parameters and aflatoxin accumulation. Food Microbiology, 25(2): 324-334.
6. Chaieb, K., H. Hajlaoui, T. Zmantar, A. B. Kahla-Nakbi, M. Rouabhia, K. Mahdouani and A. Bakhrouf. 2007. The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): a short review. Phytotherapy Research, 21(6): 501-506.
7. Evans, E. G. V. and M. D. Richardson. 1989 Medical Mycology: A Practical Approach (The Practical Approach Series). Oxford: IRL Press, pp: 235-259.
8. Guan, W., S. Li, R. Yan, S. Tang and C. Quan. 2007. Comparison of essential oils of clove buds extracted with carbon dioxide and other three traditional extraction methods. Food Chemistry, 101(4): 1558- 1564.
9. Heidtmann-Bemvenuti, R., G. L. Mendes, P. T. Scaglioni, E. Badiale-Furlong and
L. A. Souza-Soares. 2011. Biochemistry and metabolism of mycotoxins: A review. African Journal of Food Science, 5(16): 861-869.
10. Omidbeygi, M., M. Barzegar, Z. Hamidi and H. Naghdibadi. 2007. Antifungal activity of thyme, summer savory and clove essential oils against Aspergillus flavus in liquid medium and tomato paste. Food Control, 18(12): 1518-1523.
11. Paster, N., M. Menasherov, U. Ravid and B. Juven. 1995. Antifungal activity of oregano and thyme essential oils applied as fumigants against fungi attacking stored grain. Journal of Food Protection, 58: 81-85.
12. Razzaghi-Abyaneh, M., M. Shams-Ghahfarokhi, M. Kawachi, A. Eslamifar, O. J. Schmidt, A. Schmidt, A, Allameh and T. Yoshinari. 2006. Ultrastructural evidences of growth inhibitory effects of a novel biocide, Akacid® plus, on an aflatoxigenic Aspergillus parasiticus. Toxicon, 48(8): 1075-1082.
13. Soliman, K. M. and R. I. Badeaa. 2002. Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food and Chemical Toxicology, 40(11): 1669-1675.
14. Tajkarimi, M. M., S. A. Ibrahim and D. O. Cliver. 2010. Antimicrobial herb and spice compounds in food. Food Control, 21(9): 1199-1218.
15. Xu, B. J., X. Q. Jia, L. J. Gu and C. K. Sung. 2006. Review on the qualitative and quantitative analysis of the mycotoxin citrinin. Food Control, 17(4): 271-285.
16. Yahyazadeh, M., R. Omidbaigi, R. Zare and H. Taheri. 2008. Effect of some essential oil on mycelial growth of Penicillium digitatam sacc. World Journal of Microbiology and Biotechnology, 24(8): 1445-1450.
Veterinary Research & Biological Products
Volume 30, Issue 2
May 2017
Pages 147-152

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History

  • Receive Date: 07 December 2015
  • Revise Date: 10 July 2016
  • Accept Date: 26 July 2016

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