Genotypic identification of genes Encoding Capsule and F1 fimbria in Escherichia coli isolated from Feces of broilers in Kerman province

Document Type : Full Research Paper

Authors

1 Department of Microbiology, Sirjan Branch, Islamic Azad University, Sirjan, Iran

2 Department of Microbiology, Faculty of Veterinary Medicine, Shahid-Bahonar University, Kerman, Iran

3 Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran

Abstract

Escherichia coli bacteria are as a normal flora in intestine of mammals and birds, and most strains are not pathogenic while some strains can cause different disease such assepticemia, peritonitis, salpingitis, synovitis, omphalitis, coligranuloma and yolk sac infection. The purpose of this research was to identify genes coding for capsule and fimbriae F1 in E. coli strains isolated from feces of broiler chickens in Kermanprovince. A total of 60 samples were collected from the feces of broilers from the veterinary faculty of Kerman. These isolates were confirmed by phenotypic methods like culture and biochemical tests. Then multiplex PCR was performed to identify fimH, kpsMT III, PAP A,ibeA and PAPEF genes. Out of 60 isolates, 50 isolates (83.33%) amplified ‎fimH, 12 (20%) amplified ‎kpsMT III, 3 (5%) amplified ‎PAP A, 2 (3.3%) amplified ‎ibeA and 11 isolates (18.33%) amplified PAP EF.Multiplex PCR method is a powerful and effective method to detect very specific adhesion-encoding operon and other virulence factors of E. coli strains causing infection.

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 2
May 2017
Pages 101-106

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History

  • Receive Date: 07 April 2016
  • Revise Date: 26 June 2016
  • Accept Date: 10 July 2016

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