Molecular identification of Mycobacterium avium subspecies paratuberculosis 316F and III & V strains by a multi-approach strategy

Document Type : Full Research Paper

Authors

1 Tuberculin & Mallein Research and Production Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

2 Veterinary Research Department, Isfahan Agriculture and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

3 Aerobic Bacteria Veterinary Vaccines Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

4 Tuberculin & Mallein Research and Production Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

5 Aerobic Bacteria Veterinary Vaccines Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

For almost half a century Mycobacterium avium subspecies paratuberculosis (MAP) 316F and MAP III & V strains have been used in preparation of paratuberculin at Razi institute. In order to characterize their genomic properties, a multi-approach strategy was employed to authenticate their identity (PCR-IS900 or PCR-F57), their type (PCR-Collins, PCR-gyrA or PCR-gyrB) and their genotype (MLVA-Thibault or SSR-Amonsin). Consequently, both strains proved to be MAP and from a cattle type. In MLVA the genotype was found to be INMV2, a type identical to that of the French MAP 316F Merial sub-strain. In explanation of these observations, the identical patterns of both studies strains in MLVA as well as SSR typing might be a case of coincidence or a an indication of a mutual ancestral clone. Besides, the fact that the supplier of MAP 316F strain studied here is the Turkish Etlik institute, it is likely that the original source for this strain is French. Application of the strategy developed in this study on indigenous MAP isolates will improve the current molecular epidemiology understanding of paratuberculosis in Iran.

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 2
May 2017
Pages 89-100

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History

  • Receive Date: 14 February 2016
  • Revise Date: 30 May 2016
  • Accept Date: 08 June 2016

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