Effects of magnesium sulfate administration on ischemia-reperfusion kidney in rat

Document Type : Full Research Paper

Authors

1 Veterinary Graduate Student, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of Veterinary Clinical Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran

4 Department of Pathology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

This study designed to determine protective effect of magnesium sulfate on kidney function followed by ischemia-reperfusion in rat. A total 25 Wistar male rat randomly divided into 5 experimental groups (n=5). Control group: had no received medicine and gavage water for 1 week, then peritoneum opened and left kidney nephrectomy was created. The IR group: not received medicine until ischemia-reperfusion, the peritoneum opened and the renal ischemia done using clamp for 45 min, then 8 h later left nephrectomy was done. Group 3: received magnesium sulfate (25 mg/kg) for 1 week, then peritoneum opened and the renal ischemia done using clamp for 45 min, then 8 h later left nephrectomy was done. Groups 4 and 5 were similar to group 3, except rats received 50 and 100 mg/kg of magnesium sulfate, respectively. At the end of the study, tissue samples collected for histopathological evaluation and malondealdeide, Superoxide dismutase and Glutathione peroxidase levels. According to the results, the necrosis and degeneration of the nephrons was lower in magnesium sulfate treated groups compared to control grouse. Also, administration of magnesium sulfate (100 mg/kg) significantly normalized the elevated levels of malondealdeide and increased Superoxide dismutase and Glutathione peroxidase levels (P<0.05). These results suggest magnesium sulfate diminished ischemia-reperfusion injury in kidney in rat.

Keywords

References

1.Afanas' ev, I. B., Suslova, T. B., Cheremisina, Z. P., Abramova, N. E. and L. G. Korkina. 1995. Study of antioxidant properties of metal aspartates. Analyst. 120 (3): 859-862.
2.Agarwal, A., Sharma, R. K., Desai, N. R., Prabakaran, S., Tavares, A. and E. Sabanegh. 2009. Role of oxidative stress in pathogenesis of varicocele and infertility. Urology. 73(3): 461-469.
3.Beyer, W. F. and I. Fridovich. 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Analytical biochemistry. 161(2): 559-566.
4.Defraigne, J. O., Pincemail, J., Detry, O., Franssen, C., Meurisse, M. and R. Limet. 1995. Variations of glutathione and vitamin E concentrations after hypothermic storage in Euro-Collins solution and reperfusion of the rabbit kidney. Transplantation Proceedings. 27(5): 2783-2785.
5.Eckert, P. and K. Schnackerz. 1991. Ischemic tolerance of human skeletal muscle. Annals of Plastic Surgery. 26(1): 77-84.
6.Elin, R. (1987): Assessment of magnesium status. Clinical Chemistry. 33(11):1965-1970.
7.Fellstrom, B., Akuyrek, L. M. and L. Zezina. 1998. Post ischemic reperfusion injury and allograft arteriosclerosis. Transplant Proc. 384278-4280.
8.Freedman, A. M., Atrakchi, A. H., Cassidy, M. M. and W. B.Weglicki. 1990. Magnesium deficiency-induced cardiomyopathy: protection by vitamin E. Biochemical and Biophysical Research Communications. 170(3): 1102-1106.
9.Freedman, A. M., Mak, I. T., Stafford, R. E., Dickens, B. F., Cassidy, M. M., Muesing, R. A. and W. B.Weglicki. 1992. Erythrocytes from magnesium-deficient hamsters display an enhanced susceptibility to oxidative stress. American Journal of Physiology-Cell Physiology. 262(6): C1371-C1375.
10.Hans, C. P., Chaudhary, D. P. and D. D. Bansal. 2003. Effect of magnesium supplementation on oxidative stress in alloxanic diabetic rats. Magnesium research. 16(1): 13-19.
11.Holcberg, G., Amash, A., Sapir, O., Hallak, M., Sheiner, E., Ducler, D., Katz, M. and M. Huleihel. 2006. Different effects of magnesium sulfate and angiotensin II on the capacity of the fetal and maternal compartments of normal human placenta to secrete TNF-α and IL-6. Journal of reproductive immunology. 69(2): 115-125.
12.Jaeschke, H. and A. Farhood. 2002. Kupffer cell activation after no-flow ischemia versus hemorrhagic shock. Free Radical Biology and Medicine. 33(2): 210-219.
13.Kurokawa, K., Fine, L. G. and S.Klahr. 1982. Renal metabolism in obstructive nephropathy. Semin Nephrol. 231-39.
14.Liaño, F., Junco, E., Pascual, J., Madero, R. and E. Verde. 1998. The spectrum of acute renal failure in the intensive care unit compared with that seen in other settings. The Madrid Acute Renal Failure Study Group. Kidney international. Supplement. 66S16-24.
15.Liu, D. L., Jeppsson, B., Hakansson, C. H. and R. Odselius. 1996. Multiple-system organ damage resulting from prolonged hepatic inflow interruption: Electron microscopic findings. Archives of Surgery. 131(4): 442.
16.Modi, K. S., Schreiner, G. F., Purkerson, M. L. and S. Klahr. 1992. Effects of probucol in renal function and structure in rats with subtotal kidney ablation. J Lab Clin Med. 120310-317.
17.Montagna, G., Hofer, C. G. and A. M. Torres. 1998. Impairment of cellular redox status and membrane protein activities in kidneys from rats with ischemic acute renal failure. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 1407(2): 99-108.
18.Nishikimi, M., Rao, N. A. and K. Yagi. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochemical and biophysical research communications. 46(2): 849-854.
19.Oldenburg, O., Qin, Q., Krieg, T., Yang, X.-M., Philipp, S., Critz, S. D., Cohen, M. V. and J. M. Downey. 2004. Bradykinin induces mitochondrial ROS generation via NO, cGMP, PKG, and mitoKATP channel opening and leads to cardioprotection. American Journal of Physiology-Heart and Circulatory Physiology. 286(1): H468-H476.
20.Paller, M. S., Hoidal, J. and T. F. Ferris. 1984. Oxygen free radicals in ischemic acute renal failure in the rat. Journal of Clinical Investigation. 74(4): 1156.
21.Palm, F., Cederberg, J., Hansell, P., Liss, P. and P. O. Carlsson. 2003 Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension. Diabetologia. 46(8): 1153-1160.
22.Perdrizet, G. A., Giles, D. L., Dring, R., Agarwal Jr, S. K., Khwaja, K., Gao, Y. Z., Geary, M., Cowell, V. L., Berman, M. and R. Brautigam. 2006. Major hepatic trauma: warm ischemic tolerance of the liver after hemorrhagic shock. Journal of Surgical Research. 136(1): 70-77.
23.Rotruck, J., Pope, A., Ganther, H., Swanson, A., Hafeman, D. G. and W.Hoekstra. 1973. Selenium: biochemical role as a component of glutathione peroxidase. Science. 179(4073): 588-590.
24.Seth, P., Kumari, R., Madhavan, S., Singh, A. K., Mani, H., Banaudha, K. K., Sharma, S. C., Kulshreshtha, D. K. and R. K. Maheshwari. 2000. Prevention of renal ischemia–reperfusion-induced injury in rats by picroliv. Biochemical pharmacology. 59(10): 1315-1322.
25.Sheridan, A. M. and J. V.Bonventre. 2001. Pathophysiology of ischemic acute renal failure. 1327-21.
26.Taheri, Y. A., Neshat, M., Garjani, A. and N.Y. Doustar. 2012 Study the effects of metformin on renal function and structure after unilateral ischemia-reperfusion in rat. Research in Pharmaceutical Sciences. 7(5): S77.
27.Tountas, C. and R. Bergman. 1977. Tourniquet ischemia: ultrastructural and histochemical observations of ischemic human muscle and of monkey muscle and nerve. The Journal of hand surgery. 2(1): 31.
28.Unal, D., Yeni, E., Erel, O., Bitiren, M. and H.Vural. 2002. Antioxidative effects of exogenous nitric oxide versus antioxidant vitamins on renal ischemia reperfusion injury. Urological Research. 30(3): 190-194.
29.Vesey, D. A., Cheung, C., Pat, B., Endre, Z., Gobé, G. and D. W. Johnson. 2004. Erythropoietin protects against ischaemic acute renal injury. Nephrology Dialysis Transplantation. 19(2): 348-355.
30.Yamakawa, Y., Takano, M., Patel, M., Tien, N., Takada, T. and G. B. Bulkley. 2000. Interaction of platelet activating factor, reactive oxygen species generated by xanthine oxidase, and leukocytes in the generation of hepatic injury after shock/resuscitation. Annals of Surgery. 231(3): 387.
31.Yavuz, Y., Mollaoglu, H., Yürümez, Y., Ucok, K., Duran, L., Tünay, K. and L. Akgün. 2013. Therapeutic effect of magnesium sulphate on carbon monoxide toxicity-mediated brain lipid peroxidation. European review for medical and pharmacological sciences. 1728-33.
 
Veterinary Research & Biological Products
Volume 30, Issue 2
May 2017
Pages 63-75

Files

History

  • Receive Date: 07 July 2016
  • Revise Date: 11 August 2016
  • Accept Date: 15 August 2016

Share

How to cite

Statistics