Molecular identification of Ornithobacterium rhinotracheale in wildlife birds of Khouzestan province

Document Type : Full Research Paper

Authors

1 Mayahi, M., Clinical Sciences Department, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Gharibi, D., Pathobiology Department, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

3 Ghadimipour, R., Pathobiology Department, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran, Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran.

4 Talazadeh, F., Clinical Sciences Department, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract

Ornithobacterium rhinotracheale (ORT) is known as one of the involved organisms in respiratory diseases of different species of birds. So far, numerous reports have been presented about the isolation of the bacterium from commercial poultry flocks in Iran. This study was designed for molecular identification and evaluation of antimicrobial susceptibility of ORT bacterium in wildlife birds in Khouzestan province. After collection of 23 tracheal swab samples, four (17.3%) strains of ORT bacterium were isolated based on cultural methods; then three (13%) isolates were confirmed by application of specific primers. All strains isolated in this research were from Falconiformes (21.4%), which indicates the high rate of ORT isolation than that of other understudy birds (P<0.05). According to the results of determination of drug-resistance patterns of the isolates by disk diffusion method, all isolates (100%) were found sensitive to enrofloxacin, penicillin, streptomycin, cephalexin, lincospectin, tylosin, flumequine, florfenicol and tetracycline and 66.6% of them were resistant to fosfomycin, sultrim, nalidixic acid and gentamicin. In comparison with studies on strains isolated from commercial flocks especially poultry, strains detected in wild birds were sensitive to most antimicrobial agents.

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 2
May 2017
Pages 18-26

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History

  • Receive Date: 17 April 2016
  • Revise Date: 16 July 2016
  • Accept Date: 17 July 2016

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