Molecular typing and phylogenic analysis of the LPS1-8 genotypes of Pasteurella multocida isolates from poultry by LPS- PCR typing method

Document Type : Full Research Paper

Authors

1 MSC Student, Department of Medical Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organisation (AREEO), Tehran, Iran.

2 Jabbari, A. R., (Corresponding Author) Pasteurella Research National Lab Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organisation (AREEO), Tehran, Iran.

3 Section Central Lab and Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organisation (AREEO), Tehran, Iran.

4 MSC Student Jenetic, Group Molcular and Cellular, Bio Science Faculty, Kharazmi University, Tehran, Iran.

Abstract

Pasteurella multocida is a gram-negative bacteria of veterinary importance which has divided into 16 somatic or lipopolysaccharide (LPS) serovars using an agar gel diffusion precipitation test. In this study LPS PCR typing method was used for molecular typing and phylogenic analysis of the LPS1-8 genotypes of P. multocida isolates from poultry in Iran.In this study 30 isolates P. multocida were cultured on BHI medium, genomic DNA was extracted by boiling method, strains were identified by biochemical and molecular methods. LPS genotyping were typed using the LPS -PCR and Specific Primers, products were sequenced and compared with the GenBank sequences. All of the isolates were typed using the LPS PCR typing . 60% of isolates contained LPS1, 13.4% LPS2, 16.6% LPS3, 6/66% L1, L2, L3, 3/33% L2, L3. None of the other genotypes (L4-L8) were detected among the isolates. There were found considerable differences of nucleotide LPS genes of Iranian isolates and the related sequences in the GenBank. LPS- PCR typing showed that three LPS genotypes of L1, L2 and L3 were present among Iranian P. multocida isolates. This study showed that LPS- PCR typing was a suitable typing method for differentiating among avian P. multocida isolates based on LPS genotypes.

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 2
May 2017
Pages 10-17

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History

  • Receive Date: 19 June 2016
  • Revise Date: 16 July 2016
  • Accept Date: 16 July 2016

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