Capability of dietary supplementation of composite toxin binder in reducing aflatoxin B1 toxicity and its immunomodulation in rainbow trout (Oncorhynchus mykiss) fingerlings

Document Type : Full Research Paper

Authors

1 MSc Graduate of Fisheries Science, Department of Fisheries, Faculty of Natural Resources, Urmia University, I.R. of Iran.

2 Assistant Professor, Department of Fisheries, Faculty of Natural Resources, Urmia University, I.R. of Iran.

3 Associate Professor, Urmia Lake Research Institute, Urmia University, I.R. of Iran.

4 Assistant Professor, Department of Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, I.R. of Iran.

Abstract

In the present study, the effects of aflatoxin B1 (AFB1) and a compound binder on blood cell count, biochemical indices of serum and liver histology of rainbow trout was investigated. In this regard, total number of 414 trout fingerlings (9.67±1.20g) were randomly allocated into six different treatments comprising various dietary inclusion levels of AFB1 (0, 25 and 50 ppb) and binder (0 and 5%) for 60 days. At the end of experiment, blood indices did not differ amongst treatments. The highest and lowest levels of ALP activities were observed in treatments fed diets containing 5% binder-25 ppb AFB1 and the group without any dietary aflatoxin and binder inclusions, respectively (P<0.05). Activities of AST and ALT were only affected by the binder to the extent that its presence led to lower enzymes activity (P<0.05). Interaction of AFB1 and binder significantly affected total protein and globulin content (P<0.05). The albumin content was only affected by the binder (P<0.05). According to the results AFB1 reduced HSI (P<0.05). Blood vessels dilation, cytoplasmic degeneration, blood congestion and hepatic immune cells infiltration were observed in fish fed aflatoxin-contaminated diets which were severe in 50 ppb AFB1-recieved groups. In conclusion, The AFB1 resulted in liver tissue damage and despite improving the immune indices; binder did not show remarkable hepato-protective effects.

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 1
March 2017
Pages 173-182

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History

  • Receive Date: 05 April 2016
  • Revise Date: 11 June 2016
  • Accept Date: 12 June 2016

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