Effect of extender and artificial insemination on reproductive efficiency of ewes inseminated with frozen sperm

Document Type : Full Research Paper

Authors

1 Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

2 The Breeding Station of Zandi Sheep, Pishwa, Varamin, Iran.

3 Department of Poultry Science, Faculty of Agriculture, University of Tarbiat Modares. Tehran, Iran.

4 Animal Science Research Institute of Iran, Karaj, Iran.

Abstract

The aim of this study was to determine the effect of freezing medium and artificial insemination method on reproduction efficiency of Zandi ewes inseminated with frozen semen. One hundred and sixty Zandi ewes (3-4-years old, 55 kg weight) received CIDR for 12 days and at the time of CIDR removal received 400 IU of eCG and were artificially inseminated 54 h after CIDR removal. The ewes divided into two insemination groups of vaginal and trans-cervical, then, each group was inseminated with frozen sperms in extenders containing egg yolk or soybean lecithin. The results showed that the extender didn’t have any effect on sperm viability, semen lipid peroxidation and reproductive performance (P>0.05), but the artificial insemination method had a significant (P<0.05) effect on reproductive performance and pregnancy rate, parturition rate and lambing rate, which were higher in trans-cervical method than in vaginal method. In conclusion, regarding to the advantages of plant-origin extenders for ram semen freezing, soybean lecithin may be used as a suitable extender and fertility rate would be improved by trans-cervical artificial insemination method.

Keywords


1. Aboagla, E.M.E. and T. Terada. 2004. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology, 62: 1160-1172.
2. Amirat, L., D. Tainturier, L. Jeanneau, C. Thorin, O. Gérard, J.L. Courtens et al. 2004. Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl, a commercial egg yolk extender. Theriogenology 61: 895-907.
3. Anel, L., M. Kaabi, B. Abroug,M. Alvarez, E. Anel, J.C. Boixo et al. 2005. Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay. Theriogenology 63: 1235-1247.
4. Armstrong, D.T. and G. Evans. 1984. Intrauterine insemination enhances fertility of frozen semen in superovulated ewes. Journal of Reproduction and Fertility 71: 89-94.
5. Ayad, V.J., S.T. Leung, T.J. Parkinson and D.C. Wathes. 2004. Coincident increases in oxytocin receptor expression and EMG responsiveness to oxytocin in the ovine cervix at oestrus. Animal Reproduction Science 80: 237-250.
6. Bielfeld, P., R. Jeyendran, W. Holmgren and L. Zaneveld. 1990. Effect of egg yolk medium on the acrosome reaction of human spermatozoa. Journal of Andrology 11: 260.
7. Bousseau, S., J. Brillard, B. Marquant-Le Guienne, B. Guerin, A. Camus and M. Lechat. 1998. Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluents. Theriogenology 50: 699-706.
8. Ehling, C., P. Wirth, L. Schindler, K.G. Hadeler, H.H. Dopke, E. Lemme et al. 2003. Laparoscopical intrauterine insemination with different doses of fresh, conserved, and frozen semen for the production of ovine zygotes. Theriogenology 60: 777-787.
9. Esterbauer, H. and K.H. Cheeseman. 1990. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Enzyme 186: 407-421.
10. Fair, S., J.P.Hanrahan, C.M. O'Meara, P. Duffy, D. Rizos, M. Wade et al. 2005. Differences between Belclare and Suffolk ewes in fertilization rate, embryo quality and accessory sperm number after cervical or laparoscopic artificial insemination. Theriogenology 63: 1995-2005.
11. Forouzanfar, M., M. Sharafi, S. Hosseini, S. Ostadhosseini, M. Hajian, L. Hosseiniet al. 2010. In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen. Theriogenology 73: 480-487.
12. Khalifa, R.M., B.L. Sayre and G.S. Lewis. 1992. Exogenous oxytocin dilates the cervix in ewes. Journal of Animal Science 70: 38-42.
13. Lightfoot, R.J. and S. Salamon. 1970. Fertility of ram spermatozoa frozen by the pellet method. I. Transport and viability of spermatozoa within the genital tract of the ewe. Journal of Reproduction and Fertility 22: 385-398.
14. Maxwell, W. and P. Watson. 1996. Recent progress in the preservation of ram semen. Animal Reproduction Science 42: 55-65.
15. Salamon, S. and W. Maxwell. 1995. Frozen storage of ram semen I. Processing, freezing, thawing and fertility after cervical insemination. Animal Reproduction Science 37: 185-249.
16. Salamon, S. and W. Maxwell. 2000. Storage of ram semen. Animal Reproduction Science 62: 77-111.
17. Sayre, B.L. and G.S. Lewis. 1996. Cervical dilation with exogenous oxytocin does not affect sperm movement into the oviducts in ewes. Theriogenology 45: 1523-1533.
18. Sayre, B.L. and G.S. Lewis. 1997. Fertility and ovum fertilization rate after laparoscopic or transcervical intrauterine artificial insemination of oxytocin-treated ewes. Theriogenology 48: 267-275.
19. Sharafi, M., M. Forouzanfar, S.M. Hosseini, M. Hajian, S. Ostadhosseini, L. Hosseini et al. 2009. In Vitro Comparison of Soybean Lecithin Based-Extender with Commercially Available Extender for Ram Semen Cryopreservation. International Journal of Fertility and Sterility 3: 149-152.
20. Stellflug, J.N., M.C. Wulster-Radcliffe, E.L. Hensley, E.A. Cowardin, R.C. Seals and G.S Lewis. 2001. Oxytocin-induced cervical dilation and cervical manipulation in sheep: effects on laparoscopic artificial insemination. Journal of Animal Science 79: 568-573.
21. Thun, R., M. Hurtado and F. Janett. 2002. Comparison of Biociphos-Plus and TRIS-egg yolk extender for cryopreservation of bull semen. Theriogenology 57: 1087-1094.
22. Verberckmoes, S., I. De Pauw, A. Van Soom, G. Vanroose, H. Laevens and A. De Kruif. 2001. Cervical insemination in sheep. Vlag Dierge Tijd 70: 475-480.
23. Wulster-Radcliffe, M.C., B.A.Costine and G.S. Lewis. 1999. Estradiol-17 beta-oxytocin-induced cervical dilation in sheep: application to transcervical embryo transfer. Journal of Animal Science 77: 2587-2593.