Effect of extender and artificial insemination on reproductive efficiency of ewes inseminated with frozen sperm

Document Type : Full Research Paper

Authors

1 Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

2 The Breeding Station of Zandi Sheep, Pishwa, Varamin, Iran.

3 Department of Poultry Science, Faculty of Agriculture, University of Tarbiat Modares. Tehran, Iran.

4 Animal Science Research Institute of Iran, Karaj, Iran.

Abstract

The aim of this study was to determine the effect of freezing medium and artificial insemination method on reproduction efficiency of Zandi ewes inseminated with frozen semen. One hundred and sixty Zandi ewes (3-4-years old, 55 kg weight) received CIDR for 12 days and at the time of CIDR removal received 400 IU of eCG and were artificially inseminated 54 h after CIDR removal. The ewes divided into two insemination groups of vaginal and trans-cervical, then, each group was inseminated with frozen sperms in extenders containing egg yolk or soybean lecithin. The results showed that the extender didn’t have any effect on sperm viability, semen lipid peroxidation and reproductive performance (P>0.05), but the artificial insemination method had a significant (P<0.05) effect on reproductive performance and pregnancy rate, parturition rate and lambing rate, which were higher in trans-cervical method than in vaginal method. In conclusion, regarding to the advantages of plant-origin extenders for ram semen freezing, soybean lecithin may be used as a suitable extender and fertility rate would be improved by trans-cervical artificial insemination method.

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 1
March 2017
Pages 148-153

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History

  • Receive Date: 15 May 2016
  • Revise Date: 28 May 2016
  • Accept Date: 28 May 2016

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