The effects of butachlor on indicators of pathological contaminants chronic kidney tissue and muscle in goldfish (Carassius auratus)

Document Type : Full Research Paper

Authors

1 Marine Ecology Graduate Student, Faculty of Marine Biology Sciences, University of Mazandaran.

2 Faculty of Marine Biology, College of Marine Sciences, University of Mazandaran, Babolsar, Mazandaran.

3 Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.

Abstract

In order to determine the damage and histological alteration, evaluation of the effects of materials and chemical contaminants on organisms, is important. The present study reports an overall assessment of histopathological changes in kidney and muscle of goldfish in exposure to Butachlor. The experiment was carried out in1393. For this purpose, 32 adult fish supply and 4 aquariums (8 fish per tank) were exposed to different concentrations of pollutants: sub-lethal concentrations of 0.1, 0.14 and 0.28 ml butachlor 60% per liter and a control aquarium for two weeks. Then, 6 pieces was randomly selected from each treatment, the center of both kidneies and muscle tissues were removed from the bottom of the dorsal fin. At the end of the exposing period, the muscles below the dorsal fin were removed and fixed by bouins solution, processed, sectioned at 6µ thickness and further stained by Hematoxylin-Eosin general method. Histological findings in this study indicated pathological changes such as increase in Melanomacrophage centers, glomeruli shrinkage and Bowman's space expansion, reduction or blockage of the urinary tract, glomerular capillary dilation, thickening of the glomerular basement membrane, degeneration of urinary cells and separation from cell membrane, permeation of leukocytes, bleeding of urinary tubules in kidney and necrosis. Increasing the concentrations of butachlor in different treatments led to the increased extent and severity of lesions. Based on the histological study, histopathological changes in muscle, such as changes in striation, nuclear changes, cloudy swelling, hyaline degeneration, granular degeneration and necrosis were observed. The most damage was observed at the concentration of 0.28 ml/l. 

Keywords

References

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Veterinary Research & Biological Products
Volume 30, Issue 1
March 2017
Pages 89-99

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History

  • Receive Date: 10 November 2015
  • Revise Date: 04 May 2016
  • Accept Date: 19 May 2016

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