Ghrelin localization in testicular and epididymis tissues of Ram

Document Type : Full Research Paper

Authors

1 Sadegh Vaziri, S., MSc, Poultry Physiology, Islamic Azad University Branch Sanandaj.

2 Asistant Professor Islamic Azad University Branch Sanandaj.

3 MSc in Poultry Physiology, University of Kurdestan.

Abstract

The aim of the present study was to immunohistochemical positioning of ghrelin in testicular and epididymis tissues of Kurdish ram. Ghrelin, a 28-amino acid acylated peptide is an important factor involved in most of the metabolic and hormonal signals which adapt the reproductive functions in conditions of altered energy balance. This study investigated the immunohistochemical localization of ghrelin in testicular and epididymis tissues of Kurdish ram, using mouse monoclonal anti ghrelin antibody as primary antibody and polyclonal donkey anti IgG Horseradish Peroxidase (HRP) antibody as secondary antibody. Samples of testis collected from three native male sheep aged of 1.5 to 2 years old, and preserved in 10% formalin for posterior inclusion in paraffin. Histological sections with 5 micron in thickness were prepared for IHC. Immunoreactions was assessed for Germ cells, Leydig and Sertoli cells and head of epididymis. It is believed that the site of ghrelin expression in the spermatogenesis process, Leydig, sertoli cells and head of epididymis may indicate its role in local (autocrine / paracrine) regulations. This is one of the first studies to provide molecular evidence for the presence of ghrelin within the entire testicular and epididymis tissues of ram.

Keywords

References

1. Barreiro, M.L., Gaytan, F. Caminos, J.E. Pinilla, L. Casanueva, F.F. Aguilar, E. Dieguez, C. (2002). Cellular Location and Hormonal Regulation of Ghrelin Expression in Rat Testis. Journal. Biology of Reproduction. Vol, 67. pp: 1768–1776.
2. Barreiro, M.L., Tena-Semper, M.T. (2004). Ghrelin and reproduction: a novel signal linking energy status and fertility? Molecular and Cellular Endocrinology. Vol, 226. pp: 1–9.
3. Falken, Y. (2013). Effect of Endogenous and Exogenous Ghrelin on Gastrointestinal Function in Rat and Human. PhD thesis, department of clinical sciences, Karolinska Institute, Stockholm, Sweden. pp:1-60.
4. Ishikawa, T., Hitoshi, F. Takeshi, I. Atsushi, T. Masato, F. (2007). Ghrelin Expression in Human Testis and Serum Testosterone Level. Journal of Andrology. Vol. 28. No. 2. pp:320-4
5. Kheradmand, A., Omid, D. Masoud, A. Bahram, R. (2012). Ghrelin modulates testicular germ cells apoptosis and proliferation in adult normal rats. Biochemical and Biophysical Research Communications Vol. 419. pp: 299–304.
6. Lukasyk, A., Ludmila, R., Piotr, S., Aldona, K., Karolina, O., Marcin, Rucinski., Marek, R., Jerzy, S. (2009). Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium. Journal of Folia Histochemica Et Cytobiologica. Vol. 47, No. 3. pp: 5-423.
7. Lukasyk, A., Malgorzata, K., Anna, J., Aldona, K., Marcin, R., Karolina, S., Agnieszka, Z., Piotr, S., Marek, R. (2012). Expression of ghrelin receptor (GHSR-1a) in rat epididymal spermatozoa and the effects of its activation. Reproduction biology Vol. 12, Pp. 293-300.
8. Marie-Felix, A. (2010). Circulating Ghrelin Concentrations During the Transition Period of Dairy Cattle and the Associated Relationship with Milk Production. Thesis, Veterinary Science, the university of Arizona.
9. Miller, D.W., Joanne, L.H., Yvonne, A.B., Una, D., Alanna, L., Clare, L.A., Richard, G.L. (2005). Immunohistochemical evidence for an endocrine/paracrine role for ghrelin in the reproductive tissues of sheep. Journal of Reproductive Biology and Endocrinology Vol. 3, No. 60. pp:1-14.
10. Moretti, E., Vindigni, C., Tripodi, S.A., Mazzi, L., Nuti, R., Figura, N., Collodel, G. (2013). Immunolocalisation of ghrelin and obestatin in human testis, seminal vesicles, prostate and spermatozoa. Andrologia. pp. 1–7.
11. Sakata, I., Takafumi, S. (2011). The Gut Peptide Hormone Family, Motilin and Ghrelin. Book. Update on Mechanisms of Hormone Action – Focus on Metabolism, Growth 4 and Reproduction. pp.4-14.
12. Sato, T., Nakamura, Y. Shimura, Y. Ohgusu, H. Kangawa, K. Kojima, M. (2011). Structure, regulation and function of ghrelin. Journal of Biochemistry. Vol. 151. No. 2. pp: 119-128.
13. Tena-Sempere, M. (2008). Ghrelin and reproduction: ghrelin as novel regulator of the gonadotropic axis. Vitamins and Hormones. Vol. 77. pp: 285-300.
14. Yakan, B. (2011). A Concise Review: Ghrelin and Reproductive System. Erciyes Medical Vol. 33, No.4. pp:317-320.
Veterinary Research & Biological Products
Volume 30, Issue 1
March 2017
Pages 75-81

Files

History

  • Receive Date: 22 March 2016
  • Revise Date: 20 May 2016
  • Accept Date: 28 May 2016

Share

How to cite

Statistics