An investigation on histological changes of the Ross broiler’s proventriculus at different ages

Document Type : Full Research Paper

Author

Department of Anatomical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Abstract

As the proventriculus plays an important role in food digestion and there are differences in morphological and functional characteristics among different avian species, this study was carried out. A total of 54 female and males Ross broiler chicks at age 3, 7 days and then at weekly intervals until week 8 were used. The proventriculus were collected and fixed in 15% buffered formalin solution. Histologic sections of 5µm of thickness were obtained and they were stained routinely with hematoxylin-eosin and special stainings: Alcian blue, Orcein, Masson’s trichrome, PAS and Gomori’s method for reticular fibers. The results showed that by age increasing, the height of mucosal folds (particularly anastomoses), lymphatic aggregations, the number and frequency of superficial mucosal glands, the size and number of deep mucosal glands, thickness of muscularis mucosa, tunica muscularis (particularly inner circular) and serosa (particularly adipose tissue) were also increased. The shape of the deep glands was varied but polygonal, elliptical and triangular structures more prominent. The duct branching of these glands were increased with age. There was not a significant histological difference between male and female chickens. Our observations on the general histological structures of proventriculus were similar to native chickens stated in literature, but there were also some differences. The muscularis mucosa is thick and continuous. The deep glands are compound tubular and located in the lamina propria. Lymphatic aggregations are present within the connective tissue between the compound glands, and beneath their central unique ducts.

Keywords

References

1- Azad, A. 1995. Histomorphological study of proventriculus and ventriculus of native chickens (Gallus domesticus) at different ages of growth. D.V.M. thesis. Shahid Chamran University. Ahvaz, Iran.
2- Banks, W. J. 1996. Histologia veterinary aplicada. 2nd ed. pp. 629. Manual Moderno, México.
3- Bezuidenhout, A. J. and G. Van Aswegen. 1990. A light microscopic and immunocytochemical study of the grastrointestinal tract of the ostrich (Struthio camelus L.). Onderstepoort Journal of Veterinary Research 57 (1): 37-48.
4- Bozorgmehri fard, M. H., R. Morshed and H. Hosseini. 1995. Poultry diseases. 1st ed. pp. 277, 607. Kosar Agricultural Investment Co, Iran.
5- Catroxo, M. H. B., M. A. I. Lima and C. E. M. P. D. M. Cappellaro. 1997. Histological aspects of the stomach (proventriculus and gizzard) of the Red- capped cardinal (Paroaria gularis gularis). Revista chilena de anatomia 15 (1): 19-27.
6- Chikilian, M. and N. B. DE Speroni. 1996. Comparative study of digestive system of three species of tinamou. I. Crypturellus tataupa, Nothoprocta cinerascens, and Nothura maculosa (Aves: Tinamidae). Journal of Morphology 228: 77-88.
7- Dellmann, H. D. and J. Eurell. 1998. Textbook of veterinary histology. 5th ed. Lippincott Williams & Wilkins, Baltimore.
8- Jain, D. K. 1996. Histomorphology and proteolytic activity in the gastric apparatus of frugivoros, carnivoros and omnivorous species of birds. Acta Biologica Academiae Scientiarum Hungaricae 27: 135-45.
9- Junqueira, L. C. and J. Camerio. 2003. Basic Histology. 10th ed. McGraw-Hill Co, USA.
10- Kiernan, J. A. 2008. Histological and Histochemical Methods: Theory and Practice. 4th ed. pp. 144-164. Scion, Bloxham.
11- Klem, J. R. D., C. R. Brancato and J. F. Catalano. 1992. Gross morphology and general histology of the esophagus, ingluvies and proventriculus of the House Sparrow (Passer domesticus). Proceedings of the Pennsylvania Academy of Science. Pennsylvania, USA. Volume 56, pp. 141-146.
12- McLelland, J. 1979. Digestive system. pp. 637, In: J. J. Baumel, A. S. King, A. M. Lucas, J. E. Breazile and H. E. Evans (ed.), Nomina Anatomica Avium . Academic Press, London.
13- Mobini, B. 2015. General Veterinary Histology. 1st ed. pp. 52. Shahrekord Islamic Azad University, Shahrekord, Iran.
14- Piersma, T., A. Koolhaas and A. Dekinga. 1993. Interactions between stomach structure and diet choie in Shorebirds. Ornithological Advances 110: 552-564.
15- Porcescu, G. S. 2007. Comparative morphology of the digestive tract of the Black African ostrich, hen and turkey. National Council for Acreditation and Attestaion (NCAA). PhD thesis. Kishinev, Russia.
16- Rezaian, M., A. Sodagar Amiri, M. Ebrahim Akbari and F. Ebrahimpoor. 2007. Modification of fixation in preparing avian tissues. Veterinary Journal (Pajouhesh & Sazandegi) 74: 2-8.
17- Rocha, D. O. S. and M. A. I. De Lima. 1998. Histological aspects of the stomach of burrowing owl (Speotyto cunicularia). Revista chilena de anatomía 16 (2): 191-197.
18- Rossi, J. R., S. M. Baraldi-Artoni, D. Oliveria, C. D. Cruz, V. S. Frano and A. Sagula. 2005. Morphology of glandular stomach (Ventriculus glandularis) and muscular stomach (Ventriculus muscularis) of the partriged Rhynchotus rufescens. Ciência Rural 35: 6, 1319-1324.
19- Singh, S. P. 1973. Morpho-histological observations on the proventriculus in some birds viz., Ploceus phiipplnus, Upupa epops; Megalaima haemacephala and Cinnyris asiatica. Zoologischer Anzeiger 191: 45-50.
20- Thomson-Dale, S. 1999. Histogensis of the proventricular summucosal gland of the chick as revealed by light and electron microscopy. Ohio Journal of Science 69 (2): 74-84.
21- Vial, J. D., J. Garrido, M. Dabike and F. Koenig. 1997. Muscle protenins and the changes in shape of avian oxynticopeptic cells in relation to secretion. Anatomical Record 194: 293-310.
22- Yamamoto, Y., R. Kubota, Y. Atojim and S. Yoshitaka. 1996. Structure of the perilobular sheath of the deep proventricular gland of the chicken: presence and possible role of my ofibroblasts. Cell and Tissue Research 285 (1): 109-117.
 
Veterinary Research & Biological Products
Volume 30, Issue 1
March 2017
Pages 69-74

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History

  • Receive Date: 15 December 2015
  • Revise Date: 09 May 2016
  • Accept Date: 19 May 2016

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