The Effect of Unilateral Cryptorchidism on the Macroscopic and Microscopic Structure of Accessory Sex Glands in Adult Donkey: a Morphological and Histochemical Study

Document Type : Full Research Paper

Authors

1 Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz.

2 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz.

Abstract

The aim of this study was to evaluate the histological and histochemical alterations of accessory sex glands in condition of unilateral cryptorchidism. Three adult healthy donkeys and three adult donkeys with unilateral cryptorchidism were selected and euthanized. Accessory sex glands were separated and were prepared for biometric studies. Microscopic studies were done on formalin fixed and paraffin embedded tissue samples stained by Hematoxylin & Eosin (H&E) and Periodic acid Schiff (PAS) methods. Histochemical detection of lipid profiles was evaluated on frozen sections of tissue samples by Oil Red O and Sudan Black B methods. Histological alterations of the ampulla of ductus deference in animals with unilateral cryptorchidism were seen as trabecular thickness decrement, reduction of trabecular branch, decrease in secretory cells height and increase in glandular lumen diameter. In these animals, some structural changes were seen in prostate gland as mucosal folds height reduction and in bulbourethral gland as slight decrement in secretory alveoles and increment of trabecular thickness. Cellular alterations were not seen in comparison with control group. There was no indication of lipid droplets in histochemical studies in the experimental groups. The results showed that unilateral cryptorchidism leads to slight reduction in the functions of accessory sex glands, especially in intrainguinal ampulla of ductus deference.

Keywords

References

1. Abou-Elmagd, A. and Wrobel, K.H. (1989). The periurethral glandular complex in water buffalo. An ultrastructural, histological and lectin-histochemical study. Arch. Histol. Cytol. 52:501-512.
2. Absalan, F., Movahedin, M. and Mowla, S. J. (2008). Assessment of testis histological changes and sperm parameters in experimentally–induced unilateral and bilateral cryptorchid mouse model. Iranian Journal of Reproductive Medicine .6(3): 143-148.
3. Alaa, S.A., Ahmad, O.S. and Aziza, A. S. (2012). Histological and Histochemical Studies on the Ampulla of the Deferent Duct of Donkey (Equus asinus). J.Vet.R. 2: 261-270.
4. Alaa, S.A., Ahmad, O.S. and Aziza, A. S. (2013) Histomorphological Studies on the Prostate Gland of Donkey Equus asinus during Different Seasons. J.Histol .1-19.
5. Amselgruber, W. and Feder, F.H. (1986). Light and electron microscopic studies of the seminal vesicular gland (Glandula vesicularis) of the bull. Anat Histol Embryol. 15(4):361-379.
6. Aupperlel H., Gerlach K.C.P., Sabine B. and Schoonl, H.A. (1999). Histopathologicail flindings in the cryptorchidism testes of stallions. Pferdeheilkunde 6:515-522.
7. Banks, W.J. (1993). Male reproductive system. St. Louis. Baltimore. Boston. Chicago. London. Philadelphia. Sydney.Toronto: Mosby Year book.
8. Bergh, A. and Soder, O.(2007) Studies of cryptorchidism in experimental animal models. Acta Paediatr .96: 617–621.
9. Cormack, D.H.(1997). The male reproductive system. In essential histology. 1st ed. Lippincott-Ravanen Publishers. Philadelphia. New York.
10. Cox, J.E. (1982). Factors affecting testis weight in normal and cryptorchid horses. J. Rprod .Fertil.32: 129-1 34.
11. Coryn M., Moor A. and Bouters, R. (1981). Clinical, morphological and endocrinological aspects of cryptorchidism in the horse. Theriogenology, 16:489-496.
12. David, F., Penson, J. A., Lugg, C. C., Farshid, S., Andrew, L. F., Nestor, F. G.C. and Jacob, R. (1997).Effect of cryptorchidism on testicular histology in a naturally cryptorchid animal model. J. Urolo. 158 (5): 1978-1982.
13. Davies, M.C.G. (2003). Equine reproductive physiology, Breeding and Stud management. 2nd ed. CABI publishing.
14. Dutta, S., Joshi, K.R., Sengupta, P. and Bhattacharya, K. (2013). Unilateral and bilateral cryptorchidism and its effect on the testicular morphology, histology, accessory sex organs and sperm count in Laboratory Mice. J. Hum. Reprod. Sci. 6(2): 106–110.
15. Hejmej, A., Biliñska, B. (2008). The effects of cryptorchidism on the regulation of steroidogenesis and gap junctional communication in equine testes. Endokrynol Pol. 59 (2): 112–118.
16. Kaleva, M. and Toppari, J.(2005). Cryptorchidism: an indicator of testicular dysgenesis? Cell Tissue Res. 322: 167–172.
17. Marei, H.E.S., El-Habback,H.A. and Abou-Esa, K.F.(2004). Histology, fine structure and carbohydrate histochmistry of the epithelium of the accessory male genital glands of the Egyptian balady goat. Vet Med J Giza. 52:165-182
18. Mendis, H.S.M., Kerr, J.B. and Kretser, D.M.(1990). Experimental cryptorchidism in the adult mouse: II. A hormonal study. J. Androl. 11:548–54.
19. Narasimha, V. K., Madhuri, E,. Sadak, B. S. Radha, M. Y.R., Guru S. M. and Bhaskar, M. (2009). Alterations in carbohydrate metabolism under cryptorchid condition in albino rats. Journal of Applied and Natural Science. 1(1): 62-65.
20. Pinart, E., Bonet, S. and Briz, M .( 2002). Histochemical study of the interstitial tissue in scrotal and abdominal boar testes. Vet J. 163: 68–76.
21. Saalu, L. C., Adesanya, A. O., Oyewopo, A.O. and Raji, Y.(2007). An evaluation of the deleterious effect of unilateral cryptorchidism on the contralateral normally descended testis. Scientific Res and Essay. 2 (3): 074-078.
22. Sengupta, P. (2013). The laboratory rat: Relating its age with human's. Int. J. Prev. Med. 4(6):624–630.
23. Sirigu, P., Turno, F., Usai, E. and Perra, M.T. (1993). Histochemical study of the human bulbourethral (Cowper's) glands. Andrologia. 25(5):293-299.
24. Stickle, R.L. and Fessler, J.F. (1978). Retrospective study of 350 cases of equine cryptorchidism. J.W. 172:343-346.
25. Tsukise, A., Yamada, K., 1981. The histochemistry of complex carbohydrates in the scrotum of the boar. Histochemistry. 72(4):511-521.
26. Venkatarami, R. K., Geethanjali. N., Dhananjaya, R.Y., Reddanna, P. and Govindappa, S. (1983). Effect of induced bilateral cryptorchidism on the carbohydrate metabolism of reproductive tissues in albino rats. Arch Int Physiol Biochim. 91(5):405-10.
27. Virtanen, H.E., Bjerknes, R. and Cortes. D. (2007). Cryptorchidism: classification, prevalence and long-term consequences. Acta Paediatr. 96: 611–616.
Veterinary Research & Biological Products
Volume 30, Issue 1
March 2017
Pages 51-58

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History

  • Receive Date: 18 November 2015
  • Revise Date: 09 March 2016
  • Accept Date: 12 April 2016

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