Experimental study of the effect of metformin on corneal burn ulcer healing in rabbit

Document Type : Full Research Paper

Authors

1 Department of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Veterinary Graduate Student, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of Veterinary Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran;

Abstract

This study was conducted to examine the effect of metformin on corneal burn wound healing in rabbit. In this study, 20 adult healthy male New Zealand rabbits were used with an average weight of 2.5-3 kg. After general anesthesia, corneal ulcer in the left eye of the all animals was induced. After returning from anesthesia, the rabbits were randomly divided into 4 groups of 5 numbers. Then the first group was gavaged with 50 mg/kg of metformin, the second group with 100 mg/kg and the third group with 200 mg/kg while the fourth group was gavaged with water for 21 days. In the day 21 after making a corneal ulcer, tissue samples were taken and evaluated histopathologically. According to the pathological results, the vascularization, inflammation and edema of matrix of cornea significantly diminished while the epithelium of cornea increased in metformin-treated rabbits compared to control group (P<0.05). Also, administration of metformin significantly improved the natural structure of cornea collagen fibers (P<0.05). Based on the results, it seems metformin has favorable effect on the healing process in rabbit’s corneal ulcer.

Keywords

References

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Veterinary Research & Biological Products
Volume 29, Issue 3 - Serial Number 112
September 2016
Pages 110-117

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History

  • Receive Date: 07 January 2016
  • Revise Date: 19 June 2016
  • Accept Date: 14 February 2016

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