Biomechanical evaluation of three-dimensional biological scaffold made from Ostrich tendons as graft

Document Type : Full Research Paper

Authors

1 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract

The main goal of tissue engineering design and create a structure similar to the natural structure of a tissue in vivo for repair tissue damages. Tissue transplantation, there are always problems, including a shortage of suitable tissue and immunological rejection of grafted tissue is in tissue engineering is one of the newest methods to reduce the immune response to transplanted tissue, is tissue decellularization methods (Acellular). After fixation and routine tissue  process, to evaluate the structure of tissue H&E stained, collagen quality assessment xenograft Picrosirius red stained , review and tracking the decellularization, detected  nuclei trace and any DNA remaining 4', 6'diamino-2-phenylindole-2HCl (DAPI) staining was used. Assess tissue strength by biomechanical test was performed. Biomechanical results by Student t-Test and Mann-Whithney (P≤0.05)was performed with SPSS software. Compared with ostrich tendonsand  matrix three-dimensional structure after the process of decellularization, the tissue density is somewhat reduced cohesion between collagen fibers were observed in some places a few miles away. Quality collagen stained by Picrosirius red that there is no difference between them. In assessing the amount of de-cells after staining DAPI 99% of the three-dimensional scaffold and remains free of nuclear DNA. Biomechanical testing of Stress, there was no difference in graft tissue but strain showed a significant decrease. Evaluation of properties of three-dimensional tissue structure made of scaffolding and comparison with ostrich tendons found this graft is the appropriate for graft.

Keywords

References

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Veterinary Research & Biological Products
Volume 29, Issue 2
June 2016
Pages 64-74

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History

  • Receive Date: 09 September 2015
  • Revise Date: 06 April 2016
  • Accept Date: 30 November 2015

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