Molecular identification of plasmid virulence genes spv in Salmonella enteritidis isolated from poultry samples in Saveh

Document Type : Full Research Paper

Authors

1 MSc Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran.

2 Assistant Professor, Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran.

Abstract

Salmonella bacteria belonging to Enterobacteriaceae family, is important in terms of biochemical and serological aspects which imposes very diverse and important losses to humans and other animals. Salmonella enteritidis is one of the three serovars which is important and dominant in most countries of the world. Spv virulence plasmids are large and closed plasmids seen only in certain Salmonella. This virulence plasmid contain genes which give rise to virulence, serum resistance, cell growth and adhesion, as well as increasing the survival capacity of the bacteria in the intestinal tissues of infected hosts. The aim of this study is to identify genes spv position of spvB, spvC and spvR of Salmonella enteritidis isolated from poultry samples. In this cross-sectional study, 55 cases of Salmonella enteritidis from chicken samples were collected from the Saveh area and cultured on the specific medium which were confirmed by biochemical tests. DNA extraction and PCR test kits was performed to detect spv plasmid genes in the samples. The results showed that out of 55 cases of Salmonella enteritidis, 18 samples (32.7%) were spvR gene and 16 samples (29.1%) were spvB gene, and the spvC gene was not detected in any of the samples. The high prevalence of bacterial virulence genes of spvR and spvB was fully associated with the role of these genes in the pathogenesis. Salmonella can cause genetic changes in plasmid transfer between different species.

Keywords

References

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Veterinary Research & Biological Products
Volume 29, Issue 2
June 2016
Pages 2-7

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History

  • Receive Date: 09 August 2015
  • Revise Date: 06 April 2016
  • Accept Date: 23 October 2015

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