Immunohistochemical Localization of Aromatase in Goat Testicular Tissue and Accessory Glands

Document Type : Full Research Paper

Authors

1 Assistant Professor, Department of Animal Sciences, Faculty of Agriculture, University of Kurdistan, Iran

2 Graduated Master of Animal Science, Faculty of Agriculture, University of Kurdistan, Iran.

Abstract

The aim of the present study was to immunohistochemical localization of Cytochrome P450 Aromatase in male goat reproduction system. Aromatase is a terminal enzyme which transforms androgens in to estrogens. This study investigated the immunohistochemical localization of aromatase in native male goat testis, efferent ductules of epididymis, vesicle seminal and prostate glands using polyclonal anti aromatase antibody as primary antibody and anti-mouse IgG (HRP) Polyclonal antibody as secondary antibody. Samples of testis and  accessory glands collected from  five native male goat (2-4 years old), and  for IHC test were kept in Bowen solution. Then paraffin block and histological section for IHC test were prepared. Immunoreactions was observed in stages of spermatogenesis, Leydig cells, efferent ductules of epididymis, cytoplasm of vesicle seminal and prostate glands cells. The results show that aromatase in testicular cells and sexual glands converts androgens to estrogens and may be locally estrogens have a paracrine and autocrine activates in reproductive tract.

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References

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Veterinary Research & Biological Products
Volume 28, Issue 4 - Serial Number 4
December 2015
Pages 83-89

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History

  • Receive Date: 21 January 2015
  • Revise Date: 22 November 2015
  • Accept Date: 22 May 2015

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