Isolation of Cr,Cd resistant Streptomyces from marine waters and sediments in different regions in east of Guilan province

Document Type : Full Research Paper

Authors

1 Department of Microbiology, Islamic Azad University, Lahijan Branch,Lahijan.

2 Department of Microbiology, Islamic Azad University, Lahijan Branch, Lahijan

3 Department of Marine Biology, Islamic Azad University, Lahijan Branch,Lahijan.

Abstract

Expansion of industrial activities, mining and metal plating could cause releasing amount of high heavy metals into seawater and marine sediment led to heavy metals resistant microorganisms. Due to important role of Streptomyces in production of secondary metabolites, enzymes and elements reduction ability, were studied isolation of heavy metals (cadmium and chromium) resistant Streptomyces from marine sediments and coastal waters of Caspian Sea. Marine sediments and water samples were collected from coastal locations aseptically. SCA and KUA were used for the isolation of Streptomyces and biochemical testes like lipid, casein, starch hydrolysis and etc. carried out for identification of Streptomyces and determination of heavy metals (Cr ,Cd) resistant Streptomyces was performed with well diffusion assay and for detection of MIC and MBC used Microdilution assay. Also, amounts of heavy metals in different parts of coastal waters measured with atomic absorption spectrophotometry assay. A total of 41 Streptomyces isolated from coastal locations 7 isolated (17/07%) showed chromium resistant to 300mg/l, 5 isolated (12/19%) showed cadmium resistant to 600mg/l. There was a significant difference between Streptomyces, temperature, pH and salt concentration (P < 0.01). Due to existence of heavy metals (Cd,Cr) resistant Streptomyces in the Caspian Sea probability can use them as well suited agents for Bioremediation.

Keywords

References

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Veterinary Research & Biological Products
Volume 28, Issue 3 - Serial Number 3
September 2015
Pages 28-34

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History

  • Receive Date: 21 December 2012
  • Revise Date: 23 August 2015
  • Accept Date: 22 May 2014

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