Investigation of morbidity and antibacterial resistance of campylobacter spp. Sample isolation from broilers slaughter in Rafsanjan city using basic culture method

Editorial

Authors

1 Doctor of Veterinary Medicine. Islamic Azad University and Membrane Juvenile Research Club, Shahrekord Branch, Shahrekord, Iran.

2 Associated Prof. Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

3 Assistant Prof. Department of Food Hygienic, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

Abstract

Campylobacter is one of the main potentials for causing diarrhea and gastroenteritis in human saityand. One of the main sources of these bacteria is meat. Method and material: 100 samples of broiler’s meat which were slaughtered in slaughter halls all over Rafsanjan city were collected during the first 5 months of the year 1389. This study was conducted to determine the prevalence and antimicrobial resistance of Campylobacter spp; Isolated from poultry meat slaughtered in Rafsanjan slaughter houses. During this study, 31 samples (31%) were discovered to be infected and after passing chemical analysis 19 samples (61.29%) were recognized to be Campylobacter jejuni and 12 samples (38.71%) were declared Campylobacter coli. Antibacterial sensitity tests revealed that these bacteria are most susceptible to Erythromycin and Ciprofloxacin but resistant to Gentamycin and Co-trimoxazole. This study showed a high contamination of broiler’s meat to the campylobacter which is consumed as a food source. And as favas concerned about the zoonotic nature of this bacteria, it is expected to have a better attention over this matter.

1- Attanassova, V. and Ring, C. (1999) Prevalence of camoglobacter spp in poultry and meat in Germany.  International Journal of Food Microbiology ,Vol, 51. pp: 187-190##
2- Hussain, I., Shahid, M.M., Akhtar, M. and Ahrur, K. (2007) Prevalence of campylobacter species in meat, milk and other food commodities in Pakistan. Food Microbial Journal,Vol,24.pp:219-222.##
3- Insook, S., Englen Mark, D., Berrang Mark, E., Fedorka-Cray Paula, J. and Harrison Mark, A. (2007) Prevalence of Arcobacter and Campylobacter on broiler carcasses during processing. International Journal of Food Microbiology, Vol,113, No,1.pp:16-22.##
4- Jamshidi, A., Bassami, M.R. and Farkhondeh, T. (2008) Isolation and identification of campylobacter Spp. And campylobacter coli from poultery carcasses by conventional culture method and multiplex PCR in Mashhad, Iran. Iranian journal of veterinary research,shiraz university ,Vol,9, No,23.pp:132-137.##
5- Johannessen, G.S., Johnsen, G.Q., Kland, M., Cudjoe, K.S. and Hofshagen, M. (2007) Enumeration of thermotolerant campylobacter spp. from poultry carcasses at the end of the slaughter-line. Letters in Applied Microbiology, Vol,44.pp: 92-97.##
6- Kambakhsh, A. (1998) Prevalence of campylobacter Spp. (jejuni and coli) isolated from poultery carcasses slaoughtered in Tehran poultery slaughterhouse,Factulty of veterinary medecin D.V.M.,Tehran university.pp:89-92. [Farsi]##
7- Khaki, P., Yosefi, J., Rohi, P.,  Safavi, S. and Moradi, Y. (1996) Detection and identification of campylobacter coli from poultery carcasses slaughtered, Abstract book of 4th  zoonoses congress Tehran,Iran.pp:81-85. [Farsi]##
8- Koneman Elmer, W. and Stephan Allen, D. ( 1997) Color atlas and text book of diagnostic microbiology,4th edition Philadelphia-Lippincott, PP: 200-250.##
9- Luangtongkum, T., Morishita, T.Y., Ei-tayeb, A.B., Ison, A.J. and Zhang, Q. (2007) Comparison of antimicrobial susceptibility testing of campylobacter spp by the agar dilution and the agar disk diffusion methods. Journal of Clinical Microbiology ,Vol,45,No,2.pp: 590-594.##
10- Mokhtarian, H., Mohsenzadeh, M., Ghahramani, M., Moshki, M. and Fani, M.J. (2009) Detection and identification of campylobacter coli from poultry carcasses slaoughtered in Gonabad poultery slaughterhouse. Ofogh-e-Danesh.GMUHS Journal, Vol,15, No,3.pp:30-36. [Farsi]##
11- Nachamkin, I. (2003) Manual of clinical microbiology، 8th edition. ASM Press. Washington, D.C.pp: 716-722.##
12- Sáenz, Y. M., Zarazaga, M., Lantero, M.J., Gastanares, F. and Torres, C. (2000) Antibiotic resistance in Campylobacter strains isolated from animals, foods, and humans in Spain in 1997 – 1998. Antimicrobial agents and chemotherapy, Vol ,44. pp: 267 – 271##
13- Sharma, H., Unicomb, L., Forbes, W., Djordjevic, S., Valcanis, M., Dalton, C., et al. (2003) Antibiotic resistance in Campylobacter jejuni isolated from humans in the Hunter Region, New South Wales. Commun Dis Intell,Vol,27.pp: 80–8.##
14- Skirrow, M.B., Jones, D.M., Suteliffe, E. and Benjamin, J. (1993) Campylobacter bacteraemia in England and wales, Epidemiol Infect Journal,Vol,110.pp:567-73.##
15- Soltan dallal, M.M., Sanaei, M., Taremi, M., Moezardalan, S., Edalatkhah, H., Azimirad, M., et al. (2009) Prevalence and Antimicrobial resistance patten of thermophilic campylobacter Spp. (jejuni and coli) isolated from beef and raw chicken in Tehran,Iran ،Journal of Zanjan University of Medical Sciences, Vol,17, No,68.pp:85-92.[Farsi]##
16- Talebian, S.R. (2000) Prevalence of campylobacter Spp. (jejuni and coli) isolated from poultery carcasses slaoughtered in Shahrekord poultery sloughterhouse،Factulty of veterinary medecin D.V.M.,Islamic Azad university Shahrekord branch.pp:70-86. [Farsi]##
17- Talukder, K.A., Aslam, M., Islam, Z., Azmi, I.J., Dutta, D. K. and Hossain, S. (2008) Prevalence of virulence genes and cytolethal distending toxin production in campylobacter jejuni isolates from diarrheal patients in Bangladesh. Journal of Clinincal Microbiology,Vol ,46,No,4.pp:1485-1488.##
18- Thomas, A. Florion, G. and Annekathrin, F. (2005) Distribution of Campylobacter jejuni strains at different stages of a turkey slaughter kine.  Food  Microbi journal,Vol,22.pp:345-351.##
19- Van Neirop, W., Dues, A.G., Maraise, E., Aithma, N., Thothobolo, N., Kassel, M.,et al. (2005) Contamination of chicken carcasses in Gautengsouth Africa by salmonella, listeria monocytogenes and campylobacter. International Journal of Food Microbiology,Vol,99.pp: 1-6.##
20- Wieczorek, K. and Osek , J. ( 2008) Identification of virulence genes in Campylobacter jejuni and A C. coli Isolates by PCR.Bull Vet inst Pulawy journal, Vol,52.pp: 211-216.##