Evaluation of sheep meat contamination to Escherichia coli and Escherichia coli O157, O26 and O128 serotypes in Shahrekord by PCR

Editorial

Authors

1 Associate Professor, Department of Public Health and Food Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

2 Student, Faculty of Veterinary Medicine and Member of Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran

3 Student, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran

4 Lab Technician, Department of Public Health and Food Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Abstract

Data describing the microbiological status of a country’s meat products can be of great value: in acting as a de facto validation of the country’s regulatory systems, as a baseline for setting performance standards and in supporting risk assessment. O26, O45, O91, O103, O111, O113, O121, O128, O145, and O157 are the most common serotypes of enterohemorrhagic Escherichia coli associated with human disease. Considering the importance of what mentioned and the potential ability of sheep meat to transmit enterohemorrhagic E. coli to humans, the present study was conducted to evaluate sheep meat contamination to E. coli and E. coli O157, O26 and O128 serotypes in Shahrekord by PCR. Altogether 135 samples of sheep carcasses were collected from Jooneghan abattoir, Chaharmahalvabakhtiari, Iran. Samples enriched in Tryptone Soya Broth (TSB). Then, incubated broth culture was streaked onto Sorbitol MacConkey agar and MacConkey agar as selective plating media. Suspected colonies were tested by polymerase chain reaction. The contamination rate in sheep carcasses with E. coli was 50.37% (68 out of 135). 1.48% (2 out of 135) of the samples was contaminated with E. coli O128. E. coli O157 and O26 were not found in any samples. Present study suggests E. coli O157 and O26 are not prevalent in the region.

References

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Veterinary Research & Biological Products
Volume 25, Issue 4 - Serial Number 4
December 2013
Pages 38-41

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History

  • Receive Date: 21 April 2015
  • Accept Date: 21 April 2015

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