Detection of Escherichia coli O157:H7 by PCR and evaluation of resistance to beta-lactam antibiotics in Escherichia coli isolated from pigeons in Shahrekord, Iran

Editorial

Authors

1 Student, Faculty of Veterinary Medicine and Member of Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran

2 Associate Professor, Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

3 Student, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran

4 Student, Faculty of Pharmacy, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran.

Abstract

Pigeons can be carriers of some human pathogens and contribute to transmission and spread of drug resistant infectious agents to human. Considering many people’s interests to keep pigeons, present study was conducted to PCR detection of Escherichia coli O157:H7 that is responsible for outbreaks of human intestinal diseases and fatal haemolytic-uraemic syndrome worldwide and evaluation of resistance to beta-lactam antibiotics in Escherichia coli isolated from pigeons in Shahrekord. Altogether 180 samples of pigeon faeces were collected with sterile cotton swabs from different areas of Shahrekord, Iran. Swabs were placed directly into Tryptone Soya Broth (TSB). In the laboratory samples were streaked onto MacConkey agar and Cefixime Tellurite Sorbitol MacConkey agar as selective plating media. Antibiogram tests were then performed using disc diffusion method. Suspected colonies to E. coli O157:H7 were tested by polymerase chain reaction (PCR). E. coli was isolated from 93 (51.7%) of 180 the samples. Resistance of isolates to Cefotaxime, Imipenem, Cefixime, Amoxicillin, Cefalexin, Penicillin G and Oxacillin was 1.1%, 3.2% ,8.6% ,22.6% ,40.9% ,90.3% and 96.7% respectively. E. coli O157:H7 was not found in any samples. Although pigeons were not sources or carriers of E. coli O157:H7 in the region, they harbored beta-lactam antibiotics resistant E. coli and could be an important component of drugresistant infections transmission from pigeons to human, especially kids and can pose a risk to human health. Considering of what mentioned it is recommended to make pigeons owners and general public aware of potential dangers of pigeons keeping.

References

1- Apun, K., Kho, K.L., Chong, Y.L., Hashimatul, F.H., Abdullah, M.T., Rahman, M.A., Lesley, M.B., Samuel, L. (2011) Detection of Escherichia coli O157:H7 in wildlife from disturbed habitats in Sarawak, Malaysia. Research Journal of Microbiology. 6 (2): 132-139.##
2- Aşkar, Ş., Sakarya, F., Yıldırım, M. (2011) The potential risk in epizootiology of bacterial zoonozis: Pigeon (Columba livia domestica) feces. Kafkas Univ, Vet Fak Derg. 17: 1-4.##
3- Bonnedahl, J., Drobni, M., Gauthier-Clerc, M., Hernandez, J., Granholm, S., Kayser, Y., Melhus, Å., Kahlmeter, G., Waldenström, J., Johansson, A., Olsen, B. (2009) Dissemination of Escherichia coli with CTX-M Type ESBL between Humans and yellow-legged gulls in the south of France. PLoS One. 18;4(6):e5958.##
4- Bonnet, R. (2004) Growing group of extended-spectrum beta-lactamases: The CTX-M enzymes. Antimicrobial Agents and Chemotherapy. 48: 1–14.##
5- Bush, K. (2008) Extended-spectrum b-lactamases in North America, 1987–2006. Clinical Microbiology and Infection. 14: 134–143.##
6- Canto´, N.R, Novais, A., Valverde, A., Machado, E., Peixe, L., Baquero, F., Coque, T.M. (2008) Prevalence and spread of extended-spectrum b-lactamase-producing Enterobacteriaceae in Europe. Clinical Microbiology and Infection. 14: 144–153.##
7- Chen, K., Guo, W., Cheng, F., Lin, C., Lin, J., Dong, X. (2000) Investigation on E. coli O157 in Fujian, China. Zhonghua Yu Fang Yi Xue Za Zhi. 34(3): 156-8.##
8- Costa, D., Poeta, P., Saenz, Y., Vinue, L., Rojo-Bezares, B., Jouini, A., Zarazaga, M., Rodrigues, J., Torres, C. (2006) Detection of Escherichia coli harbouring extended-spectrum beta-lactamases of the CTX-M, TEM and SHV classes in faecal samples of wild animals in Portugal. Journal of Antimicrobial Chemotherapy. 58: 1311–1312.##
9- Dolejská, M., Bierosová, B., Kohoutová, L., Literák, I., Cízek, A. (2009) Antibiotic-resistant Salmonella and Escherichia coli isolates with integrons and extended-spectrum beta-lactamases in surface water and sympatric black-headed gulls. Journal of Applied Microbiology. 106(6):1941-50.##
10- Dolejská, M., Cízek, A., Literák, I. (2007) High prevalence of antimicrobial-resistant genes and integrons in Escherichia coli isolates from Black-headed Gulls in the Czech Republic. Journal of Applied Microbiology. 103(1):11-9.##
11- Elder, R.O., Keen, J.E., Siragusa, G.R., Barkocy-Gallagher, G.A., Koohmaraie, M. (2000) Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides and carcasses of beet cattle during processing. National Academy of Sciences. 97:2999-3003##
12- Ewers, C., Guenther, S., Wieler, L.H., Schierack, P. (2009) Mallard ducks – a waterfowl species with high risk of distributing Escherichia coli pathogenic for humans. Environmental Microbiology Representative. 1: 510–517.##
13- Gannon, V.P., D’Souza, S., Graham, T., King, R.K., Rahn, K., Read, S. (1997) Use of the flagellar H7 genes as a target in multiplex PCR assay and improved specificity in identification of enterohemorrhagic E. coli strains. Journal of Clinical Microbiology. 35(3): 656-662.##
14- Gaukler, S.M., Linz, G.M., Sherwood, J.S., Dyer, N.W., Bleier, W.J., Wannemuehler, Y.M., Nolan, L.K., Logue, C.M. (2009) Escherichia coli, Salmonella, and Mycobacterium avium subsp. paratuberculosis in Wild European Starlings at a Kansas Cattle Feedlot. Avian Diseases. 53: 544-551.##
15- Hawkey, P.M. (2008) Prevalence and clonality of extendedspectrum b-lactamases in Asia. Clinical Microbiology and Infection. 14: 159–165.##
16- Jay, M.T., Atwill, E.R., Cooley, M., Carychao, D., Vivas, E., Chandler, S., Orthmeyer, D., Li, X., Mandrell, R.E. (2010) Occurrence of Escherichia coli O157:H7 in wildlife populations in a major produce production region in california. 110th General Meeting of the American Society for Microbiology. San Diego, California. 23-27.##
17- Karmali, M.A., Steele, B.T., Petric, M., Lim, C. (1983) Sporadic cases of haemolytic-uraemic syndrome associated  with faecal cytotoxin and cytotoxin-producing E. coli in stools. Lancet. 1:619-20##
18- Krauss, H., Weber, A., Appel, M., Enders, B., Isenberg, H.D., Schiefer, H.G., Slenczka, W., Von Graevenitz, A., Zahner, H. (2003) Zoonoses: Infectious diseases transmissible from animals to humans. 3rd ed. Washington DC: American Society for Microbiology Press.##
19- LeJeune, J., Homan, J., Linz, G., Pearl, D. (2008) Role of the European starling in the transmission of E. coli O157 on dairy farms. Proceedings of the 23th Vertebrate Pest Conference. Published at University of California, Davis: 31-34.##
20- Literák, I., Vanko, R., Dolejská, M., Cízek, A., Karpísková, R. (2007) Antibiotic resistant Escherichia coli and Salmonella in Russian rooks (Corvus frugilegus) wintering in the Czech Republic. Letters in Applied Microbiology. 45(6):616-21##
21- Morabito, S., Dell’Omo, G., Agrimi, U., Schmidt, H., Karch, H., Cheasty, T., Caprioli, A. (2001) Detection and characterization of shiga toxinproducing Escherichia coli in feral pigeons. Veterinary Microbiology. 82: 275-283.##
22- Paton, A.W., Paton, J.C. (1998) Detection an characterization of shiga toxigenic Escherichia coli by using multiplex PCR assay for stx1, stx2, eae A, enterohemorrhagic E. coli hlyA, rfbO111 and rfbO157. Journal of Clinical Microbiology. 36: 598-602.##
23- Radimersky, T., Frolkova, P., Janoszowska, D., Dolejska, M., Svec, P., Roubalova, E., Cikova, P., Cizek, A., Literak, I. (2010) Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons. Journal of Applied Microbiology. 109(5):1687-95.##
24- Reid, C.A., Small, S.M., Avery, S., Buncic, S. (2002) Presence of  food borne pathagens on cattle hides. Food Control. 13:411-415##
25- Rice, L.B. (2009) The clinical consequences of antimicrobial resistance. Current Opinion in Microbiology. 12:476–481.##
26- Riley, I.W., Remis, R.S., Helgerson, S.D. (1983) Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine. 308:681-685. ##
27- Santaniello, A., Gargiulo, A., Borrelli, L., Dipineto, L., Cuomo, A., Sensale, M., Fontanella, M., Calabria, M., Musella, V., Menna, L.F., Fioretti, A. (2007) Survey of shiga toxin-producing Escherichia coli O157:H7 in urban pigeons (Columba livia) in the city of Napoli, Italy. Italian Journal of Animal Science. 6: 313-316.##
28- Shen, J., Song, C., Tao, J., Wang, Y., Ji, J., Luo, H. (2011) The intestinal bleeding E. coli O157:H7 animal host carrier situation survey of Xishuangbanna state. Chinese Journal of Health Laboratory Technology. 6: 1515-1516.##
29- Shere, J.A., Bartlett, K.J., Kaspar, C.W. (1998). Longitudinal Study of Escherichia coli O157:H7 dissemination on four dairy farms in wisconsin. Applied and Environmental Microbiology. 64(4): 1390-99.##
30- Silva, V.L., Nicoli, J.R., Nascimento, T.C., Diniz, C.G. (2009) Diarrheagenic Escherichia coli strains recovered from urban pigeons (Columba livia) in Brazil and their antimicrobial susceptibility patterns. Current Microbiology. 59(3):302-8.##
31- Sorum, H., Sunde, M. (2001) Resistance to antibiotics in the normal flora of animals. Veterinary Research. 32: 227–241.##
32- Stampi, S., Caprioli, A., De Luca, G., Sacchetti, R., Quaglio, P., Zanetti, F. (2004) Detection of Escherichia coli O157 in bovine meat products in northern Italy. International Journal of Food Microbiology 22:241-245.##
33- Tahmasby, H., Momtaz, H., Salehi, N., Rafiee Dolatabadi, M., Yektaneh, F. (2011) Prevalence of Escherichia coli O157:H7 in pet birds in Yazd, Iran. Pejouhandeh. 16(5):252-5.##
34- Tanaka, C., Miyazawa, T., Watarai, M., Ishiguro, N. (2005) Bacteriological survey of feces from feral pigeons in Japan. Journal of Veterinary Medical Science. 67: 951-953.##

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History

  • Receive Date: 21 April 2015
  • Accept Date: 21 April 2015

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