PCR detection of Listeria monocytogenes in bovine endometritis from Shahrekord, Iran

Editorial

Authors

1 Assistant Professor, Department of Clinical Science, Faculty of Veterinary Medicine and Member of Research Institute of Zoonotic Diseases, Shahrekord University, Shahrekord, Iran

2 Student, Faculty of Veterinary Medicine and Member of Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran

3 Student, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran

4 Assistant Professor, Department of Veterinary Reproduction and Obstetrics, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

Endometritis as a risk factor for cystic ovarian disease, anoestrus and other reproductive disorder, is characterized by impact of dairy production performance and causes serious economic losses. Listeria monocytogenes can cause endometritis. Considering the importance of what mentioned present study was conducted to detection of L. monocytogenes in bovine endometritis from Shahrekord, Iran. Altogether 180 vaginal discharge samples of bovine endometritis were collected with sterile cotton swabs from Shahrekord, Iran. The swabs were placed directly into Listeria enrichment broth. Secondly enrichments were streaked on Palcam agar. Suspected colonies were evaluated for detecting L. monocytogenes by polymerase chain reaction (PCR) method. L. monocytogenes was not found in any samples. Present study suggests absence of L. monocytogenes in bovine endometritis in the region.

References

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Veterinary Research & Biological Products
Volume 26, Issue 3 - Serial Number 3
September 2013
Pages 31-34

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History

  • Receive Date: 21 April 2015
  • Accept Date: 21 April 2015

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