Histological and developmental study of sclera and cornea in Canadian ostrich embryo (Struthio camelus)

Document Type : Full Research Paper

Authors

1 Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Professor of Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Due to the high economic value of ostrich and the farming industry to further promote increased productivity, knowledge in different fields of science, including detailed knowledge of tissue structures vital organs from this animal looks. Ostrich’s eye is the cornea closest to the human eye because of the same anatomical features of the human cornea, corneal transplantation can be used ostrich. In this study, 12 healthy fertile ostrich eggs, ostrich farms in Mashhad birch ages 22, 26, 30 and 36 days of embryonic and three-day-old chicks were collected and sent to the histology lab. Histological evaluation of the whole eye sections were stained by hematoxylin and eosin and masson trichrome. The sclera and cornea were studied at different ages in different layers. The results showed that the three main layers of the eye at embryonic day 22 (fibrosis, vascular and nerve) was formed. In addition to the scleral tissue were also observed in mesenchymal hyaline cartilage. At older ages, the cartilage thickness increased with age and was found as articulated around the eyes. There was bone tissue in anterior part of the sclera at the junction of the cornea. It was observed in age of 30 days old and was developed in older. At the age of 22 days, the corneal epithelium was composed of a thin tissue of non-keratinized stratified squamous seemed. Up to 2 to 3 layers of the epithelium layer seemed to increase with age the number of layers that have been added. Stroma was extensive and Bowman’s membrane was uncertain. Descemet membrane at 22 day of age was weakly identified and was seen as folded. Corneal endothelium was the innermost layer, which is seen as a simple cuboidal epithelium. With increasing age, the corneal epithelium was thicker and the fiber density was increased in stoma and Bowman and Descemet membranes were identified.

References


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Veterinary Research & Biological Products
Volume 27, Issue 4 - Serial Number 4
December 2015
Pages 25-31

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History

  • Receive Date: 22 June 2015
  • Revise Date: 12 September 2012
  • Accept Date: 03 April 2014

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