Detection of bluetongue virus in aborted lamb fetuses in Chaharmahal va Bakhtiari and Isfahan provinces, by RT-PCR method

Document Type : Full Research Paper

Authors

1 Master of Animal Science , Mazandaran Central Laboratory of Veterinary Organization , Sari , Iran.

2 Assistance Professor of Animal Science , Islamic Azad University , Qaemshahr Branch , Qaemshahr , Iran

3 Manager of Mazandaran Veterinary Organization , Sari, Iran.

Abstract

To consider of seroprevalence and to determine the focus of Brucellosis propagation in domestic animal population of different regions of Mazandaran Province, within 5 years (2006- 2010), blood samples of 4845 numbers of sheep and goats of different districts of the province were taken. Due to continental situations, aggregation and dispersion of domestic animal population of the province, we gathered information from ranchers who had come to veterinary networks of different districts of the province to diagnosis, treatment and the get the compensation for accession and complication of the disease. We also took blood samples from all sick animals and we did random sampling for other livestock existing around contamination focus point. After serum separation, Rose Bengal test was done for all samples as the primary recognizing test, and then Right and 2-Mercaptoethanole test were done on positive serums to make the final diagnosis. The results obtained from this research showed that 457 of all tested serum samples (9.44 %) were positive in supplementary test. Among all regions of the province, Sari district with 15.35 % of contamination, allocates the most amounts of reactor livestock to itself, and is the main focus point of disease outbreak in the province. Districts such as Noor, Nowshahr- Chaloos and Babol with 14.09, 13.02 and 12.01 percent are in next ranks of contamination, respectively. Also, at this time, Juibar district had the least contamination (0.84 %). It seems that ranchers raise awareness and prevent the import and impermissible slaughter of contaminated animals, vaccination for all domestic animals and studies its portion effect in vaccinated animals, have an important role in control and prevention of brucellosis disease in domestic animal population of Mazandaran province.  

References

- پاکزاد، پ. (1385): اصول و تفسیر آزمایشهای سرولوژی بالینی. چاپ دهم، انتشارات نور دانش، صفحه 150- 141
2- پورکریم، م. ذوقی، ا. (1387): سروباکتریولوژی بروسلوز دامی- انسانی در استان بوشهر. دوفصلنامه طب جنوب، سال دوم، 1: صفحه 58- 54
3- تاج بخش، ح. (1385): باکتری شناسی عمومی. چاپ هفتم، انتشارات دانشگاه تهران، صفحه 698- 694
4- دستورالعمل اجرایی مبارزه با بروسلوز دامی (1384)، معاونت بهداشتی و پیشگیری سازمان دامپزشکی کشور. شماره 12687/43
5- دوماری، ح. (1388): بررسی سرمی بیماری بروسلوز در گاوهای شهرستان جیرفت. مجله تحقیقات دامپزشکی، صفحه 236- 233
6- ذوقی، ا. (1368): اپیدمیولوژی بیماریهای واگیر. چاپ اول، انتشارات جهاد دانشگاهی دانشگاه تهران، صفحه 220- 202
7- قاسمی، ب. محمدیان، ب. صوفی مجید پور، م. (1382): بررسی اپیدمیولوژی بیماری بروسلوز انسانی و دامی در استان کردستان در سال 80- 1376. مجله علمی دانشگاه علوم پزشکی کردستان، سال هشتم، 2: صفحه 32- 23
 
8- Bokaie, S., Heydari Latibari, S., Abbaszadeh, S., Mousakhani, H., Rabbani, M., Sharifi, L., (2009):  Ecological study of brucellosis in humans and animals in Khoy, a mountainous District of the IR. of Iran. Iranian Journal of Microbiology. 1 (4): 14- 17
9- Corbel, M. J., (1991): Identification of dye sensitive strains of Brucella melitensis. Journal of Clinical Microbiology. 22 (5): 1066- 1068
10- Delpy, I. P., Kaveh, M., (1945): The occurrence of brucellosis in Iran. The isolation of the causative agent of contagious abortion in cattle. Review, Journal of Faculty of Veterinary Medicine of University of Tehran. 1 (1): 21- 29
11- Ellis, H., (2006): Sir David Bruce, a pioneer of tropical medicine. British Journal of Hospital medicine. 67 (3): 158
12- Eshchary, Y., Shereyer, P., Caspi, E. (1980): Brucella septicemia in pregnancy case report. Europian Journal of Opestetrics Gynecology Report. 16: 33- 51
13- Gerald, L., Mandel, E. J.,  Bannet, R. D., (2000): Principles and practice of infectious diseases. 5th edition. Churchill livingstone, New york. 2386- 2391 
14- Hasanjani Roushan, M. R., Mohrez, M., Smailnejad Gangi, S. M., Soleimani Amiri, J., Hajiahmadi, M., (2004): Epidemiological features and clinical manifestations in 469 adult patients with brucellosis in Babol, Northern Iran. Epidemiology Infection. 132: 1109- 1114
15- Jordi, S., Ramin, P., Pere, G. A., (2000): seroepidemiological study of brucellosis in a rural endemic area. Journal of Clinical Microbiology. 74- 78
16- Larsen, H., (1992): Letters from abroad to Professor dr. med. Bernhard Bang, Copenhagen. History of veterinary medicine. 17 (1–2): 1- 5
17- Nicoletti, P., (1991): Brucella. Diagnostic procedures in veterinary bacteriology and mycology. 5th edition. Lee and Febiger, London, UK. 123 - 142
18- Nielsen, K., (2002): Diagnosis of brucella by serology. Veterinary microbiology. 90: 447- 459
19- Refai, M., (2002): Incidence and control of brucellosis in the Near East region. Veterinary microbiology. 90 (1- 4): 81- 110
20- Rezaei- Sadaghiani, R., Zowghi, E., Marhemati- Khamene, B., Mahpeikar, H. A., (1996): Brucella melitensis infection in sheep- dogs in Iran. Archives of Razi Institute. 46/47: 1- 7
21- Samaha, H., Mohamed, T. R., Khoudair, R. M., Ashour, H. M., (2009): Serodiagnosis of brucellosis in cattle and humans in Egypt. Immunobiology. 214: 223- 226
22- World Health Organization. (1997): Fact sheet N173. World Health Organization, Geneva, Switzerland.
Veterinary Research & Biological Products
Volume 27, Issue 4 - Serial Number 4
December 2015
Pages 17-24

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History

  • Receive Date: 21 April 2013
  • Revise Date: 13 September 2014
  • Accept Date: 21 March 2014

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