Histopathological and biochemical study of Allium hirtifolium and Sodium Phenytoin on healing of cutaneous lesions in Common carp

Document Type : Full Research Paper

Authors

1 Department of basic Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Food Hygiene and Aquaculture, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

3 Razi Vaccine & Serum Research Institute, Mashhad Branch, Iran

Abstract

Cutaneous lesions of fish from viral, bacterial, parasitic and chemical and mechanical factors are common. The rapid progress of infection and the mortality of fish need more attention. Allium hirtiofolium is a native plant in Iran which contains saponins, it has a role in inhibiting and killing cancer cells with anti-allergenic, anti-bacterial, anti-inflammatory and anti-virus effects. In this Study 120 Common Carp (10 ± 60 g) with 3% NaCl solution was sterilized for 15 minutes. At the beginning of the test a long cut 1.5 cm and in depth of 2 mm in the rear (left side) of all of the fish was created. Afterward they were divided into 3 groups (n=40 in each), Group 1 (control), group2 (treated with5 mg / litSodium phenytoin) and group3 (treated with 5 mg / lit Allium hirtifolium). Days 0, 3, 6, 14 and 20 tests, seven fish from each group were randomly selected after that blood and tissue samples were taken from the site of the lesion. The results of the biochemical changes showed that difference in cholesterol and liver enzymes aspartate amino transferase (AST) and alanine amino transferase (ALT) in group3 compared to the control group was significant (P <0.05), in triglyceride was found a significant difference and it was higher in groups 3 and 1 (p <0.05), but No significant difference in blood glucose between groups were observed in any of the test days Between group1 and 3. Histopathology results obtained from 3 groups showed that in the group treated with phenytoin sodium healing was in less time than in group3. According to the results, further studies are recommended.

References

1- Abdou, A. Abou-Zeid, A A. Sherbeeny, MR0El and Abou-El, ZH. (1972) Antimicrobial activities of Allium sativum, Allium cepa, Raphanus sativus, Capsicum frutescens, Eruca sativa, Allium kurrat on bacteria . Plant Foods for Human Nutrition, Vol 22, pp: 129-35.
2- Ariga, T. Seki, T. (2006) Antithrombotic and anticancer effects of garlic-derived sulfur compounds: a review. Biofactors, 26(2),pp: 93-103.
3- Arvind, K. P. Akhilesh Sharma, M.B. Asha Sodanim, B.and Suman Hada, M. (1993) Topical Phenytoin in wound healing, Vol 32( 3),pp:153–228.
4-Ayatollahi, S. (1991) Ph.D Dissertation . Phyto chemical investigation. Synthesis, and kinetics of activity of the anticandidal component of Allium sativum, and a hypothesis for its mode of action, Mycologia 69, pp: 793-825.
5- Bullock, A.M.and Robert R.J. (1975) The influence of ultraviolet-B radiation on the mechanism of wound repair in the skin of the Atlantic salmon (samo salar), J. Fish. Dis. 15,pp:143-152.
6. Dill, RE. and Lacopino, AM. (1997) Myofibroblasts in phenytoin induced hyperplastic connective tissue in rat and in human gingival overgrowth. JPeriodontal, 68,pp: 375-380.
7-El-Nahas, M. Gawish, H. Tarshoby, M.and State, O. (2009). The impact of topical phenytoin on recalcitrant neuropathic diabetic foot ulceration, J Wound Care, 18(1), pp:33-7.
8-Ferguson, H.W. (2006) Systemic Pathology of Fish , 2 rd ed. Scotian Press, U, PP: 64-85.
9-Ghodrati Azadi, H. Ghaffari, S M. Riazi, G H. Ahmadian, Sh.and Vahedi, F. ( 2008) Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor
cell lines Cytotechnology, 56(3),pp: 179–185.
10-Ghodrati Azadi, H. Riazi, G H. Ghaffari, S M. Ahmadian, Sh. and Javdani Khalife, T. (2009) Effects of Allium hirtifolium (Iranian shallot) and its allicin on microtubule and cancer cell lines African Journal of Biotechnology, Vol. 8, (19), Pp: 5030-5037.
11- Hasamnis, A.A. Mohanty, B.K. Muralikrishn, A. and Patil, S. (2010) Evaluation of Wound Healing Effect of Topical Phenytoin on Excisional Wound in Albino Rats J Young Pharm, 2(1): 59–62.
12- Khaksari, M. Rezvani, ME. Sajadi, MA.Soleimani, A. (2000) The effect of topically applied water extract of
Rhazyastricta on cutaneous wound healing in rats, J Semnanuniversity of medical Science, 1(3)pp: 1-10.
13- Mittal, A.K. Munshi, J.S.D. (1974) on the regeneration and repair of superficial wounds in the skin of Rita rita(Bagridae), Acta. Anat.88,pp:424-442.
14- Modaggh, S. and Salchian, S. (1989) Use of phenytoin in healing war and non war wounds, J. Dermatol,28(3), pp:347-350.
15- Ozaki, H. Akima, R. and Harad, M. (1993) On regeneration of skin in carp,. J. Tokyo. Univ. Fish, 59, 2. pp: 69-78.
16-Quilhac, A. and Sire, J.Y. (1999) Spreading, proliferation and differatiation of the epidermis after wounding a cichlid fish(Hemishromis bimaculatus), Anatomic. Rec, 254,pp:435-451.
17- Robert, R. J. Ball, H.J. Munro, A.L.S. and Shearer,W.M. (1971) Studies on ulcerative dermal necrosis of salmonids (The healing process in fish maintained under experimental condition), J.Fish. Biol,pp: 221-224.
18- Robert, R. J. (1975). Melanin –containing cells of teleost fish and their relation to disease. In: The pathology of fish . 1st ed. W. E. Ribelin & G. Migaki. The University of Wasconsin Press. Madison, USA. pp: 399-428.
19- Roberts ,R.J.F. (1989). Pathology. 2 rd ed . WB.Saunders company. Philadelphia, USA. pp:13-15.
20- Shahsavani, D. Movassaghi, A. Baghi, L. (2005). Studying clinical and histopathological effects of phenytoin sodium on cutaneous wounds in the gold fish ( Carracius auratus). Iranian
Sci. Fisheries J. 13.pp:65-74.
21- Shrifpour, I. (2004). Experimental study on histology of circumstance of wound healing process in common carp (Cyprinus carpio ). Iranian Sci. Fisheries J. 13pp: 91-116.
22- Song, S, and Cheng, T. (1997)The effect of Systemic and Local irradiation on wound macrophages and the repair promoting action of phenytoin sodium. Chung HuaI HsuchTsa chi.; 77: 54-57.
23- Wagner, G.N. Don Stevens, E. and Harvey-clark C. (1999). Wound healing in Rainbow trout flowing surgical site preparation with a povidine – Iodine antiseptic.J.Aqua. Anim. Health.
11pp: 373-382.
Veterinary Research & Biological Products
Volume 27, Issue 4 - Serial Number 4
December 2015
Pages 10-16

Files

History

  • Receive Date: 21 March 2013
  • Revise Date: 23 September 2013
  • Accept Date: 21 April 2014

Share

How to cite

Statistics