Comparison of intradermal and intravenous injection methods for laboratory rabbit anti-sheep hemolysin production

Document Type : Full Research Paper

Author

Assistant Professor, Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Karaj, Iran.

Abstract

There are many guidelines for hemolysin production, but for production with higher titers, standardization of procedures is essential. In this study, for the first time in the country, the hemolysin was produced by whole  sheep blood intradermal injection to laboratory rabbits and was compared with the intravenous injection of 2% washed sheep red blood cells routine method. The produced hemolysins were titerd by kolmer method. In whole blood intradermal  injection method, the rabbit anti-sheep hemolysin titer was at least 1:9600 whereas in 2% washed cells intravenous injection method, obtained 1:1200. The obtained results of the three replicates were taken on two methods listed were exactly the same as before. In order to increasing the products stability, they was lyophilized and were  kept in -20oC. For survey in maintained products stability time, once every six months for 2 years, both the products, were tittered. To evaluate the in vitro activity, they were used in serological tests. In this survey, the  hemolysin produced by whole  sheep blood intradermal injection, in all cases, and even after 2 years of production, there is no reduction in titer and it was very favorable in serologic tests, but the product was prepared by intravenous injection, showed sharp decline in titer and it  was not an acceptable result in serologic tests. According to the results, the whole blood intradermal method occording to this protocol can be replaced to routingly 2% washed cells intravenous injection for production of  anti-sheep hemolysin with high titer, stability and effect. 

Keywords

References

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History

  • Receive Date: 22 December 2013
  • Revise Date: 21 April 2014
  • Accept Date: 23 October 2014

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